Proceedings of the International Conference of Tropical Studies and its Applications (ICTROPS 2022)

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The Effect of L-Ascorbic Acid-2-Phosphate on Human Adipose-Derived Stem Cells Population Doubling Time and Angiogenic Potency

Authors
Imam Rosadi1, *, Karina Karina2, 3, 4, 5, Viol Dhea Kharisma6, Arif Nur Muhammad Ansori7, 8
1Department of Biology, Faculty of Mathematics and Natural Sciences, Mulawarman University, Samarinda, Indonesia
2HayandraLab, Yayasan Hayandra Peduli, Jakarta, Indonesia
3Klinik Hayandra, Yayasan Hayandra Peduli, Jakarta, Indonesia
4Universitas Pembangunan Nasional Veteran Jakarta, Depok, Indonesia
5Pusat Unggulan Regenerative Medicine and Neurosains, Jakarta, Indonesia
6Division of Molecular Biology and Genetics, Generasi Biologi Indonesia Foundation, Gresik, Indonesia
7Faculty of Veterinary Medicine, Univeritas Airlangga, Surabaya, Indonesia
8Professor Nidom Foundations, Surabaya, Indonesia
*Corresponding author. Email: imamrosadi@unmul.ac.id
Corresponding Author
Imam Rosadi
Available Online 27 July 2023.
DOI
10.2991/978-94-6463-180-7_42How to use a DOI?
Keywords
ADSCs; L-ascorbic acid-2-phosphate; PDT; angiogenic potency
Abstract

Adipose-derived stem cells (ADSCs) have been developed as substitute cells for tissue engineering. However, in order to get a high number of cells, over-repassage leads the cells to become big in spread size indicating that senescence occurred. This study aimed to evaluate the potency of ascorbic acid to induce the proliferation and angiogenic potency of human ADSCs and inhibit the aging process of the cells. Human ADSCs were isolated by an enzymatic process. ADSCs were cultured in 10% fetal bovine serum (FBS) supplemented by ascorbic acid in various concentrations (1, 3, 6 µg/ml). The morphology, proliferation, population doubling time (PDT), and angiogenic potency were evaluated. Angiogenic potency analysis was performed using gene expression of vascular endothelial growth factor (VEGF) and its receptor (VEGFR). Ascorbic acid on 6 µg/mL suppressed the senescence proved by cell spread areas, growth curves, viability, PDT, and percentage of scratch width closure. No significant difference in vascular endothelial growth factor and its receptor expression to the control. However, the high concentration of ascorbic acid (6 µg/ml) expressed the highest VEGF and VEGFR. Our study showed that ADSCs with supplementation of ascorbic acid (6 µg/ml) promoted proliferation and angiogenic potency.

Copyright
© 2023 The Author(s)
Open Access
Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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Volume Title
Proceedings of the International Conference of Tropical Studies and its Applications (ICTROPS 2022)
Series
Advances in Biological Sciences Research
Publication Date
27 July 2023
ISBN
978-94-6463-180-7
ISSN
2468-5747
DOI
10.2991/978-94-6463-180-7_42How to use a DOI?
Copyright
© 2023 The Author(s)
Open Access
Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

Cite this article

risenwbib
TY  - CONF
AU  - Imam Rosadi
AU  - Karina Karina
AU  - Viol Dhea Kharisma
AU  - Arif Nur Muhammad Ansori
PY  - 2023
DA  - 2023/07/27
TI  - The Effect of L-Ascorbic Acid-2-Phosphate on Human Adipose-Derived Stem Cells Population Doubling Time and Angiogenic Potency
BT  - Proceedings of the International Conference of Tropical Studies and its Applications (ICTROPS 2022)
PB  - Atlantis Press
SP  - 401
EP  - 408
SN  - 2468-5747
UR  - https://doi.org/10.2991/978-94-6463-180-7_42
DO  - 10.2991/978-94-6463-180-7_42
ID  - Rosadi2023
ER  -