Proceedings of the International Conference of Tropical Studies and its Applications (ICTROPS 2022)

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Potential of Methanol Extract From Rosella (Hibiscus Sabdariffa) as an Alternative Dye in Gram Staining

Authors
Mohammad Indra Pratama1, Rajun1, Faradilla Dahlan1, Nurul Anisa1, Indri Yudita Ningsih1, Khusnul Yumni Bulan Sari1, Sri Sela Pratiwi1, Niky Aulia Putri1, Teguh Pribadi1, Didimus Tanah Boleng1, Dora Dayu Rahma Turista1, *
1Biology Education Department Faculty of Teacher Training and Education, Mulawarman University, Samarinda, Indonesia
*Corresponding author. Email: doraturistaofficial@gmail.com
Corresponding Author
Dora Dayu Rahma Turista
Available Online 27 July 2023.
DOI
10.2991/978-94-6463-180-7_32How to use a DOI?
Keywords
Methanol; Rosella; E. coli; Staphylococus; Klebsilla; Safranin; Violet Crystal
Abstract

Gram staining is one of the important steps to identify bacteria. This type of staining uses synthetic substances safranin and violet crystals which can result in environmental pollution. Rosella flowers (Hibiscus sabdariffa) contain natural dyestuffs, namely anthocyanins that have the potential to color bacterial cells. This study aims to find out whether rosella flower methanol extract (Hibiscus sabdariffa) can be used as a substitute for alternative dyestuffs in Gram staining. This study is an experimental study with a descriptive approach that uses three repetitions. The results showed that rosella flower extract (Hibiscus sabdariffa) cannot be used as an alternative to natural dyes for staining bacteria Staphylococcus aureus, Klebsiella Pneumonia and E. coli. The bacterial cell wall has a complex layer so it requires a special type of dye to be able to penetrate it. Different materials need to be carried out so that they can be used as alternative dyes in gram staining.

Copyright
© 2023 The Author(s)
Open Access
Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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Volume Title
Proceedings of the International Conference of Tropical Studies and its Applications (ICTROPS 2022)
Series
Advances in Biological Sciences Research
Publication Date
27 July 2023
ISBN
978-94-6463-180-7
ISSN
2468-5747
DOI
10.2991/978-94-6463-180-7_32How to use a DOI?
Copyright
© 2023 The Author(s)
Open Access
Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

Cite this article

risenwbib
TY  - CONF
AU  - Mohammad Indra Pratama
AU  - Rajun
AU  - Faradilla Dahlan
AU  - Nurul Anisa
AU  - Indri Yudita Ningsih
AU  - Khusnul Yumni Bulan Sari
AU  - Sri Sela Pratiwi
AU  - Niky Aulia Putri
AU  - Teguh Pribadi
AU  - Didimus Tanah Boleng
AU  - Dora Dayu Rahma Turista
PY  - 2023
DA  - 2023/07/27
TI  - Potential of Methanol Extract From Rosella (Hibiscus Sabdariffa) as an Alternative Dye in Gram Staining
BT  - Proceedings of the International Conference of Tropical Studies and its Applications (ICTROPS 2022)
PB  - Atlantis Press
SP  - 290
EP  - 297
SN  - 2468-5747
UR  - https://doi.org/10.2991/978-94-6463-180-7_32
DO  - 10.2991/978-94-6463-180-7_32
ID  - Pratama2023
ER  -