Proceedings of the 1st International Conference Postgraduate School Universitas Airlangga : "Implementation of Climate Change Agreement to Meet Sustainable Development Goals" (ICPSUAS 2017)

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FISH PROTEIN PROFILE SUBMERGE ALUM SOLUTIONBASED ON SDS-PAGE

Authors
Akhmad Mubarok
Corresponding Author
Akhmad Mubarok
Available Online August 2017.
DOI
10.2991/icpsuas-17.2018.72How to use a DOI?
Keywords
Euthynnus Affinis, alum solution, protein profile, SDS-PAGE
Abstract

Euthynnus affinis and other fish product is perishable food because have protein and water more in order to long savings and high quality so food additive one of alum solution. Alum used for high quality contents is heavy metal is Aluminium negative effect for health in particular enzymatic system and tissue. Liver and kidney first tissue impact because detoksification organ. This study aims for analysis protein profile Euthnnus Affinis fish submerge alum solution 0%, 10%, 20% dan 30% by SDS-PAGE method. Study object use Euthynnus Affinis fish submerge alum solution by concentration 0% (X0), 10% (X1), 20% (X2), dan 30% (X3) as long as 1 hour. Result this study showed sample X0 (without alum) protein band mayor cunted in 225 kDa, 139 kDa, 107 kDa, 71 kDa, 40 kDa, 36 kDa, 25 kDa, 25 kDa and minor protein 23 kDa, 23 kDa, 21 kDa, 19 Kda, 15 kDa. Sample X1 (10% Alum) showed protein band mayor cunted in 104 kDa dan 44 kDa. and minor protein 38kDa, 35 kDa, 29 kDa, 24 kDa, 22 kDa, dan 15 kDa. Sample X2 (20% Alum) and X3 (30% Alum) showed same character protein band minor 29 kDa, 24 kDa dan 22 kDa. Change sub units protein because increasing alum solution so become change band protein.

Copyright
© 2018, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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Volume Title
Proceedings of the 1st International Conference Postgraduate School Universitas Airlangga : "Implementation of Climate Change Agreement to Meet Sustainable Development Goals" (ICPSUAS 2017)
Series
Advances in Social Science, Education and Humanities Research
Publication Date
August 2017
ISBN
978-94-6252-461-3
ISSN
2352-5398
DOI
10.2991/icpsuas-17.2018.72How to use a DOI?
Copyright
© 2018, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

Cite this article

risenwbib
TY  - CONF
AU  - Akhmad Mubarok
PY  - 2017/08
DA  - 2017/08
TI  - FISH PROTEIN PROFILE SUBMERGE ALUM SOLUTIONBASED ON SDS-PAGE
BT  - Proceedings of the 1st International Conference Postgraduate School Universitas Airlangga : "Implementation of Climate Change Agreement to Meet Sustainable Development Goals" (ICPSUAS 2017)
PB  - Atlantis Press
SP  - 338
EP  - 340
SN  - 2352-5398
UR  - https://doi.org/10.2991/icpsuas-17.2018.72
DO  - 10.2991/icpsuas-17.2018.72
ID  - Mubarok2017/08
ER  -