Morphological and Molecular Characterization of Variegated-Leaf Pattern in Dendrobium ‘Burana Green’ Based on the Structure of VAR2 Gene

DOI

10.2991/978-94-6463-122-7_50How to use a DOI?

Keywords

Anatomy-Molecular; Chlorophyll; Dendrobium ‘Burana Green’; Variegated-leaf; VAR2 gene

Abstract

Some plants developed variegated pattern on their leaves, where the leaves color separated to green part that indicate normal chloroplasts and white part that indicates abnormal chloroplast. Dendrobium ‘Burana Green’ is a hybrid orchid that has variegated phenotype on its leaves, where the middle part is green and surrounded by white edges. There are several factors that can cause variegated phenotypes such as transposon activity, genetic mutations linked to pigment degradation in the nucleus and plastids, and differences in pigment distribution. This study aims to investigate the variegated phenotype of D. ‘Burana Green’ through morphology, anatomy, and molecular analysis specifically on VAR2. Our observation showed that variegated phenotype already occured from early leaf development. The ratio between green and white part of the leaf also gradually changing with the green part eventually dominates the leaf. We also found no stomata on the white part. The chloroplast number and total chlorophyll content in the green part are significantly higher. Twenty seven mutations were detected on the VAR2 isolated from the white part which suspected to contribute in variegated leaves phenotype.

Copyright

© 2023 The Author(s)

Open Access

Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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TY  - CONF
AU  - Saifa Usni Putri
AU  - Aviesta Linggabuwana
AU  - Febri Yuda Kurniawan
AU  - Muhammad Dylan Lawrie
AU  - Endang Semiarti
PY  - 2023
DA  - 2023/05/22
TI  - Morphological and Molecular Characterization of Variegated-Leaf Pattern in Dendrobium ‘Burana Green’ Based on the Structure of VAR2 Gene
BT  - Proceedings of the 3rd International Conference on Smart and Innovative Agriculture (ICoSIA 2022)
PB  - Atlantis Press
SP  - 524
EP  - 539
SN  - 2468-5747
UR  - https://doi.org/10.2991/978-94-6463-122-7_50
DO  - 10.2991/978-94-6463-122-7_50
ID  - Putri2023
ER  -