Supplementation of Mangosteen (Garcinia mangostana) Pericarp Filtrate in Tris-egg yolk-based Diluent on Buck Sperm Membrane Integrity

DOI

10.2991/icoh-17.2018.48How to use a DOI?

Keywords

AI, Antioxidant, Dilution, Mangosteen, Semen quality

Abstract

This study was conducted to evaluate supplementation of Mangosteen (Garcinia mangostana) pericarp filtrate in a tris-egg yolk-based diluent on buck sperm membrane integrity. Semen was collected from 5 bucks by artificial vagina method. Fresh semen evaluated for colour, pH, volume, concentration, mass motility, motility, viability, sperm abnormality and sperm membrane integrity. Semen was diluted with tris-egg yolk-based extender supplemented with different levels Mangosteen (Garcinia mangostana) pericarp filtrate (0, 10, 20 and 30 %) v/v with the ratio of 1 semen : 9 diluter. Semen used mass motility of 2+ and motility of 70%. Immediately after dilution semen was stored for 4øC and sperm membrane integrity percentage were observed at 0, 24, 48 h and 72 h. The obtained data were analyze with Analysis of Variant (ANOVA) and Least Significant Difference was determined. The experiment was designed using completely random design ( 4 treatments and 10 replications). The results showed that the level of mangosteen pericarp filtrate had very significant effect (P<0,01) on sperm membrane integrity percentage in 0, 24, 48 and 72 h of cooling. It concluded that the best mangsteen pericarp filtrate level for resulting optimal sperm membrane integrity was 10%.

Copyright

© 2018, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Nurul Isnaini
AU  - Nuryadi Nuryadi
AU  - Eko Nugroho
PY  - 2017/07
DA  - 2017/07
TI  - Supplementation of Mangosteen (Garcinia mangostana) Pericarp Filtrate in Tris-egg yolk-based Diluent on Buck Sperm Membrane Integrity
BT  - Proceedings of the 1st International Conference in One Health (ICOH 2017)
PB  - Atlantis Press
SP  - 246
EP  - 250
SN  - 2468-5739
UR  - https://doi.org/10.2991/icoh-17.2018.48
DO  - 10.2991/icoh-17.2018.48
ID  - Isnaini2017/07
ER  -