Proceedings of the 1st International Conference in One Health (ICOH 2017)

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Effect of Follicle Size on Metaphase II and Cleavage Rate of Goat Oocyte

Authors
Sri Wahjuningsih, Muhammad Nur Ihsan
Corresponding Author
Sri Wahjuningsih
Available Online July 2017.
DOI
10.2991/icoh-17.2018.45How to use a DOI?
Keywords
Goat Oocytes, In Vitro Maturation, In Vitro Fertilization, Follicle Size
Abstract

The aim of this research was to determine the effect of follicle size on Metaphase II and cleavage rate of Goat Oocyte. Ovaries from slaughterhouse transported to the laboratory within 2 h of slaugtering in normal saline. The ovaries were cut and classified two classes of follicle size : small (2-4 mm diameter ) and large (>4-8 mm diameter) follicles. All culture were done in ten replicates at 38.5oC, 5 % CO2, 95 % humidity. Only fully Cumulus Oocyte Complexes (COCs) and homogenous cytoplasm were used. The COCs were matured for 26 h in TCM-199 medium supplemented with FCS 10 % + EBS 10%. Chi square test were used for statistical analysis. Metaphase II rate was determined by microscope in a sample of COCs denuded,fixed and stained with aceto orcein 1 %. In vitro fertilization was performed using frozen semen (2 X 106 spermatozoa/mL in EBSS solution. The percentage of COCs reaching Metaphase II was 86.42 % (261/302) and 74.33 % (220/300) for oocytes originating from large and small diameter follicles,respectively (P<0.05). After in vitro fertilization , the developmental competence of oocytes dependently increased with follicular size. Higher cleavage rate was obtained from large follicles compare to small follicles (P<0.05).

Copyright
© 2018, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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Volume Title
Proceedings of the 1st International Conference in One Health (ICOH 2017)
Series
Advances in Health Sciences Research
Publication Date
July 2017
ISBN
978-94-6252-481-1
ISSN
2468-5739
DOI
10.2991/icoh-17.2018.45How to use a DOI?
Copyright
© 2018, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

Cite this article

risenwbib
TY  - CONF
AU  - Sri Wahjuningsih
AU  - Muhammad Nur Ihsan
PY  - 2017/07
DA  - 2017/07
TI  - Effect of Follicle Size on Metaphase II and Cleavage Rate of Goat Oocyte
BT  - Proceedings of the 1st International Conference in One Health (ICOH 2017)
PB  - Atlantis Press
SP  - 232
EP  - 234
SN  - 2468-5739
UR  - https://doi.org/10.2991/icoh-17.2018.45
DO  - 10.2991/icoh-17.2018.45
ID  - Wahjuningsih2017/07
ER  -