Proceedings of the 3rd International Conference on Biology, Science and Education (IcoBioSE 2021)

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Optimization of Multiplex PCR for Detection of Rat Meat-Contaminated Beef-Based Food Product

Authors
Afifatul Achyar1, *, Moralita Chatri1, Lisna Khairiyah S1
1Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang, Padang, Indonesia
*Corresponding author. Email: afifatul.achyar@fmipa.unp.ac.id
Corresponding Author
Afifatul Achyar
Available Online 22 May 2023.
DOI
10.2991/978-94-6463-166-1_63How to use a DOI?
Keywords
PCR multiplex; food authentication; ND5; Bos taurus
Abstract

Food safety is an important point in consuming food, in order to produce good health for the community. But lately there were many foods circulating in the community that are no longer guaranteed to be safe, namely the existence of counterfeiting and mixing beef with other meats such as rat meat. Rat meat is forbidden for Muslims to consume. In addition, rat can be a reservoir for several disease-causing pathogens in humans. This makes the majority of the Muslim community in Indonesia very worried and in dire need of guarantees for safe and halal food. The purpose of this study was to develop an in-house method for detection of rat meat-contaminated beef-based food product using the multiplex PCR method. Multiplex PCR in this study was optimized on the three variation of PCR program. The optimum multiplex PCR conditions to amplify the ND5 gene of rat and beef simultaneously was using the combination of cycling program of Mur-MA primer pair and Bos primer pair that resulting 919 bp and 206 bp DNA bands which were amplicons of therat and bovine ND5 gene, respectively.

Copyright
© 2023 The Author(s)
Open Access
Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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Volume Title
Proceedings of the 3rd International Conference on Biology, Science and Education (IcoBioSE 2021)
Series
Advances in Biological Sciences Research
Publication Date
22 May 2023
ISBN
978-94-6463-166-1
ISSN
2468-5747
DOI
10.2991/978-94-6463-166-1_63How to use a DOI?
Copyright
© 2023 The Author(s)
Open Access
Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

Cite this article

risenwbib
TY  - CONF
AU  - Afifatul Achyar
AU  - Moralita Chatri
AU  - Lisna Khairiyah S
PY  - 2023
DA  - 2023/05/22
TI  - Optimization of Multiplex PCR for Detection of Rat Meat-Contaminated Beef-Based Food Product
BT  - Proceedings of the 3rd International Conference on Biology, Science and Education (IcoBioSE 2021)
PB  - Atlantis Press
SP  - 531
EP  - 537
SN  - 2468-5747
UR  - https://doi.org/10.2991/978-94-6463-166-1_63
DO  - 10.2991/978-94-6463-166-1_63
ID  - Achyar2023
ER  -