Primers and Probes Design of Multiplex qPCR For Food Authentication Detection

DOI

10.2991/978-94-6463-166-1_33How to use a DOI?

Keywords

Food authentication detection; Multiplex qPCR; ND5; Porcine; Rat

Abstract

Indonesia continues to confront difficulties and barriers in obtaining halal certification because some unresponsible individuals continue to adulterate halal food by combining it with pork or fanged animals in order to increase their profits. Therefore, it is necessary for Halal Examining Institutions (Lembaga Pemeriksa Halal/LPH) to create techniques that can identify different types of non-halal meat in a product. The solution is multiplex quantitative real-time PCR (qPCR), which can quantitatively and concurrently detect the non-halal content of numerous animal species. The most important component that determines the optimal performance of multiplex qPCR analysis is the design of the primers and probes. In order to detect and amplify DNA from swine, rat, and bovine, this study attempts to build primers and probes that satisfy the multiplex qPCR criteria for food authentication. Using Geneious Prime software, the ND5 sequences of Sus scrofa, Rattus rattus, and Bos taurus were aligned. In contrast to designing a probe using a species-specific sequence, primers for these species were created using a conserved area. These bioinformatic analyses led to the development of two conserved primers with 166 bp amplicons and Sus, Rattus, and Bos species-specific probes.

Copyright

© 2023 The Author(s)

Open Access

Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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TY  - CONF
AU  - Moralita Chatri
AU  - Dwi Hilda Putri
AU  - Dezi Handayani
AU  - Afifatul Achyar
PY  - 2023
DA  - 2023/05/22
TI  - Primers and Probes Design of Multiplex qPCR For Food Authentication Detection
BT  - Proceedings of the 3rd International Conference on Biology, Science and Education (IcoBioSE 2021)
PB  - Atlantis Press
SP  - 249
EP  - 257
SN  - 2468-5747
UR  - https://doi.org/10.2991/978-94-6463-166-1_33
DO  - 10.2991/978-94-6463-166-1_33
ID  - Chatri2023
ER  -