Proceedings of the 3rd International Conference on Biology, Science and Education (IcoBioSE 2021)

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Effect of Honey Extender for Spermatozoa Dilution on Fertility and hatchability of Javaen Barb Fish (Systomus orphoides)

Authors
Sri Hilia1, *, Abinawanto1, Astari Dwiranti1, Anom Bowolaksono1, Fadhillah1, Retno Lestari1, Anang Kritanto2
1Departement of Biology, Faculty of Mathematics and Science (FMIPA), Universitas Indonesia, Depok, Indonesia
2Pusat Riset Zoologi Terapan OR Hayati Dan Lingkungan, Badan Riset Dan Inovasi Nasional, Jakarta, Indonesia
*Corresponding author. Email: abinawanto.ms@sci.ui.ac.id
Corresponding Author
Sri Hilia
Available Online 22 May 2023.
DOI
10.2991/978-94-6463-166-1_53How to use a DOI?
Keywords
Extender; Fertility; Javaen barb fish; Spermatozoa; Systomus orphoides
Abstract

Javaen barb fish (Systomus orphoides) population was declined due to overfishing and environmental changes. Cultivation efforts have been made, but the high sperm concentration causes a decrease in fertility. Honey extender is used to dilute sperm, maintain buffer balance, and provide nutrition for sperm. The objective of this study was to obtain the optimum concentration of honey extender on the fertility and hatchability rate of Javaen barb fish sperm. The concentrations (0%, 0.2%, 0.4%, 0.6%, 0.8%, and 1%) of honey were used. The ratio between sperm and the mixture of extenders (fish Ringer and honey) used was 1:10. Sperm and egg collection was done by stripping. Evaluation was carried out by observing of fertility and hatchability rate. The data was analysed using one-way ANOVA, followed by Tukey's test. Based on the results of the ANOVA test and Tukey's test, it was seen that there was a significant difference (P < 0.05) on the fertility and hatchability rate. The significant difference was found in hatchability rate, but not difference on fertility. Honey concentration 0.8% was the optimum concentration that produced the highest percentage hatchability (96,33 ± 6.27%) after dilution with honey extender.

Copyright
© 2023 The Author(s)
Open Access
Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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Volume Title
Proceedings of the 3rd International Conference on Biology, Science and Education (IcoBioSE 2021)
Series
Advances in Biological Sciences Research
Publication Date
22 May 2023
ISBN
978-94-6463-166-1
ISSN
2468-5747
DOI
10.2991/978-94-6463-166-1_53How to use a DOI?
Copyright
© 2023 The Author(s)
Open Access
Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

Cite this article

risenwbib
TY  - CONF
AU  - Sri Hilia
AU  - Abinawanto
AU  - Astari Dwiranti
AU  - Anom Bowolaksono
AU  - Fadhillah
AU  - Retno Lestari
AU  - Anang Kritanto
PY  - 2023
DA  - 2023/05/22
TI  - Effect of Honey Extender for Spermatozoa Dilution on Fertility and hatchability of Javaen Barb Fish (Systomus orphoides)
BT  - Proceedings of the 3rd International Conference on Biology, Science and Education (IcoBioSE 2021)
PB  - Atlantis Press
SP  - 426
EP  - 431
SN  - 2468-5747
UR  - https://doi.org/10.2991/978-94-6463-166-1_53
DO  - 10.2991/978-94-6463-166-1_53
ID  - Hilia2023
ER  -