Varietal Identification of Liberica Coffee in Kepulauan Meranti Riau using RAPD Marker: A Preliminary Study

DOI

10.2991/978-94-6463-166-1_49How to use a DOI?

Keywords

Liberica; Lim 1; Lim 2; Meranti; RAPD

Abstract

Identity of plant variety is essential for crop production system. Two liberica coffee varieties, Lim 1 and Lim 2, have been cultivated in Kepulauan Meranti where peat lands area predominantly found. In addition to their adaptability to peat lands, the two varieties also were reported resistant against Hemileia vastatrix, one of main fungal pathogens in coffee plants. Molecular-based techniques, such as Random Amplified Polymorphic DNA (RAPD) marker, are considered as reliable approaches compared to morphological-based method for identifying plant varieties. This study aimed to analyze RAPD profiles to identify Lim coffee varieties. We sampled Lim 1, Lim 2, and local coffee genotype from Kabupaten Bengkalis and extracted genomic DNA from young leaves and carried out PCR (Polymerase Chain Reaction) using three primers (OPA-04, OPE-03, OPE-18). Results of electrophoresis showed that all three primers produced polymorphic bands. RAPD profiles generated by OPE-03 and OPE-18 could dishtinguish the three different coffee varieties or genotype. In addition, two bands (1,500 and 2,500 bp) amplified by PCR with OPA-04 were specifically observed in Bengkalis coffee, while 650 bp band revealed by PCR with OPE-03 was only present in Lim 1. Based on our result, we suggest that RAPD marker is suitable for identifying liberica coffee varieties and can be used for further investigation to develop liberica varietal specific markers.

Copyright

© 2023 The Author(s)

Open Access

Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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TY  - CONF
AU  - Ninik Nihayatul Wahibah
AU  - Rizka Prathama Putri
AU  - Atria Martina
AU  - Arini Arini
AU  - Yasir Sidiq
PY  - 2023
DA  - 2023/05/22
TI  - Varietal Identification of Liberica Coffee in Kepulauan Meranti Riau using RAPD Marker: A Preliminary Study
BT  - Proceedings of the 3rd International Conference on Biology, Science and Education (IcoBioSE 2021)
PB  - Atlantis Press
SP  - 384
EP  - 391
SN  - 2468-5747
UR  - https://doi.org/10.2991/978-94-6463-166-1_49
DO  - 10.2991/978-94-6463-166-1_49
ID  - Wahibah2023
ER  -