NSE (Neurone Specific Enolase) expression of the different time points in PC12 cells to differentiate into nerve cells

DOI

10.2991/icmse-15.2015.128How to use a DOI?

Keywords

NSE expression,PC12 cells, Time points

Abstract

NSE plays a vital role in PC12 cell differentiation, expecialy the length of the synapse and the formation of microtubules. Here, NGF(Nerve Growth Factor) and OGD(oxygen-glucose deprivation) were used to stimulate PC12 cells, and convert them into neurons to establish an ischemia in vitro model. In order to further find NSE expression in PC12 cells. Western blot were used to determinate the protein expression of NSE. NSE expression was determined by western blotting after different treatment time periods. The results shown are representative of three repeated experiments. The expression was different with different time. Consult the relevant literature, rats or cell tumor cells adrenal collaterals (PC12 cells) can be NGF induction into Nerve cells, NSE exists in PC12 cell differentiation process, it and the length of the synapses and microtubules formation of closely related, the NSE in synaptic neuron growth plays an important role.

Copyright

© 2015, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Chunli Mei
AU  - Lijuan Zhang
AU  - Huimin Liu
PY  - 2015/12
DA  - 2015/12
TI  - NSE (Neurone Specific Enolase) expression of the different time points in PC12 cells to differentiate into nerve cells
BT  - Proceedings of the 2015 6th International Conference on Manufacturing Science and Engineering
PB  - Atlantis Press
SP  - 714
EP  - 718
SN  - 2352-5401
UR  - https://doi.org/10.2991/icmse-15.2015.128
DO  - 10.2991/icmse-15.2015.128
ID  - Mei2015/12
ER  -