The Production of Alkaline Protease from a Recombinant Strain Expressed Apr

DOI

10.2991/icmsa-15.2015.77How to use a DOI?

Keywords

Alkaline Protease, Recombinant, Optimization, Activity.

Abstract

The alkaline protease gene, Apr, from Bacillus licheniformis 2709 was cloned into an expression vector pET - 28b(+). The pET-28b(+) - Apr was expressed in a high expression strain E. coli BL21. In order to develop and optimize mathematical model of protease activity, three variables were investigated, including peptone concentration (6 g/L to 10 g/L), incubation time (16 h to 32 h) and agitation frequency (150 r/min to 240 r/min), by response surface methodology (RSM). The protease produced by recombinant strain with optimal protease activity was predicted to be obtained at: the peptone concentration 8.08 g/L, the incubation time 24.25 h and the agitation frequency 180.14 r/min. Under the optimal condition, the protease activity achieved 1125.81 U/mL.

Copyright

© 2015, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Mei-Shuo Zhang
AU  - Ya-Nan Yang
AU  - Xue-Nan Li
AU  - Song-Yi Lin
PY  - 2014/06
DA  - 2014/06
TI  - The Production of Alkaline Protease from a Recombinant Strain Expressed Apr
BT  - Proceedings of the 2015 International Conference on Material Science and Applications
PB  - Atlantis Press
SP  - 427
EP  - 431
SN  - 2352-541X
UR  - https://doi.org/10.2991/icmsa-15.2015.77
DO  - 10.2991/icmsa-15.2015.77
ID  - Zhang2014/06
ER  -