Immobilization of lactase on modified magnetic chitosan microspheres
- DOI
- 10.2991/icmia-16.2016.161How to use a DOI?
- Keywords
- Immobilization, Lactase, Magnetic fluid, Lactase activity
- Abstract
The immobilization of lactase is a more attractive approach to overcome the disadvantage of the free lactase. In the presented work magnetic Fe304 fluid was synthesized by chemical coprecipitation and its surface was modified with sodium oleate. Then the liquid paraffin was used as dispersant and glutaraldehyde as cross-linking agent to prepare modified chitosan magnetic microspheres with Fe3O4 as magnetic core and its outer coated with chitosan through the reversed-phase suspension polymerization method. Finally lactase was immobilized on modified chitosan magnetic microspheres by adsorption and crosslinking with glutaraldehyde. Results indicated the recovery of immobilized lactase was the highest when lactase was immobilized on the modified magnetic chitosan microspheres at 20 for 22 h and then reacted with 1.0 mL of 0.4% glutaraldehyde for crosslinking at 30 for 3 h, and a hydrolysis rate of 38% was obtained when the immobilized lactase was used to hydrolyze milk at 40 for 3h.
- Copyright
- © 2016, the Authors. Published by Atlantis Press.
- Open Access
- This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).
Cite this article
TY - CONF AU - Qian Fang AU - Shujuan Jiang AU - Yang Yang AU - Yanfeng Tuo AU - Guangqing Mu PY - 2016/11 DA - 2016/11 TI - Immobilization of lactase on modified magnetic chitosan microspheres BT - Proceedings of the 2016 5th International Conference on Measurement, Instrumentation and Automation (ICMIA 2016) PB - Atlantis Press SN - 1951-6851 UR - https://doi.org/10.2991/icmia-16.2016.161 DO - 10.2991/icmia-16.2016.161 ID - Fang2016/11 ER -