Sensitivity of Different Collagens to Proteolytic Enzyme Treatment

DOI

10.2991/ahsr.k.201001.009How to use a DOI?

Keywords

collagen, animal collagens, proteolytic enzyme

Abstract

Collagen is a ubiquitously expressed essential structural component of all metazoan extracellular matrices. It is present in organisms ranging from sponges to humans. Although the general functions of collagens are comparable, their characteristics are quite distinct. There are 19 different types of animal collagens, as defined by their tissue location. Much less is known about the structure of collagens from hydrations as compared to mammal collagens. This research aimed to compare the sensitivity of two types of Collagen; one is from hydration (silver carp, Hypophthalmichthys molitrix) and other is from mammals – (cattle, Bos taurus Taurus) to trypsin, collagenase and acidic proteases. Here we report the differential sensitivity of these two collagens to proteases and the different patterns produced by proteolytic digestion. We also show that it is possible to isolate Collagen from fish skin according to our protocol, as the purified substance is specifically cleaved by collagenase. We also show the different sensitivities of fish and cattle collagen to proteases.

Copyright

© 2020, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - V.D. Anokhova
AU  - E.G. Chupakhin
AU  - N.A. Pershina
AU  - S.A. Storublevtsev
AU  - L.V. Antipova
AU  - L.V. Matskova
AU  - S.S. Antipov
PY  - 2020
DA  - 2020/10/03
TI  - Sensitivity of Different Collagens to Proteolytic Enzyme Treatment
BT  - Proceedings of the International Conference “Health and wellbeing in modern society” (ICHW 2020)
PB  - Atlantis Press
SP  - 44
EP  - 49
SN  - 2468-5739
UR  - https://doi.org/10.2991/ahsr.k.201001.009
DO  - 10.2991/ahsr.k.201001.009
ID  - Anokhova2020
ER  -