Isolation and Cytotoxic Assay of α-amyrin from Callistemon citrinus Stem Bark Against Cervical Cancer Cells (HeLa)

DOI

10.2991/978-94-6463-431-0_15How to use a DOI?

Keywords

Cervical Cancer; C. citrinus; Cytotoxic Activity

Abstract

Cervical cancer attitudes a significant health challenge due to its high prevalence and mortality rates. As an alternative approach to treating cancer, scientists are exploring the use of bioactive compounds derived from herbal plants. One such plant is the bottle brush plant (C. citrinus). Isolation of α-amyrin from C. citrinus stem bark initiates by maceration using methanol and ethyl acetate. The phytochemical assay of C. citrinus extract contained alkaloids, terpenoids, tannins, and flavonoids. Antioxidant assay by DPPH method showed that the methanol and ethyl acetate extract had IC₅₀ = 58.03, 84.21 ppm respectively. Fractionation of methanol extract was carried out by Vacuum Liquid Chromatography and purification by Column Chromatography. The isolated compound, namely isolate M-5b2, has a white needle crystal with melting point about 213.4-215.1℃. The structure of isolate M-5b2 was determined by spectroscopy analysis (UV-Vis, FT-IR, ¹H-NMR, ¹H↔¹H COSY, and GC-MS). The analysis of spectroscopic data from M-5b2 suggests that it is an α–amyrin compound. The cytotoxic activity of α–amyrin compounds against cervical cancer cells (HeLa) by MTT method exhibited low cytotoxic by IC₅₀ value of 56.75 ppm.

Copyright

© 2024 The Author(s)

Open Access

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TY  - CONF
AU  - Melysa Saleh
AU  - Rahmat Kurniawan
AU  - Hawa Purnama Celala Ary Cane
PY  - 2024
DA  - 2024/05/30
TI  - Isolation and Cytotoxic Assay of α-amyrin from Callistemon citrinus Stem Bark Against Cervical Cancer Cells (HeLa)
BT  - Proceedings of selected papers of International Conference on Health, Science, and Environment (ICHSE 2023)
PB  - Atlantis Press
SP  - 131
EP  - 146
SN  - 2468-5747
UR  - https://doi.org/10.2991/978-94-6463-431-0_15
DO  - 10.2991/978-94-6463-431-0_15
ID  - Saleh2024
ER  -