16S rRNA Fragment of Metagenomic DNA, Antibacterial and Cellulase Activity from Garbage Enzymes of Sweet Orange (Citrus sinensis) Peels
- DOI
- 10.2991/978-94-6463-431-0_17How to use a DOI?
- Keywords
- antibacterial; cellulase activity; metagenomic DNA; 16S rRNA
- Abstract
Organic waste, such as fruit peels, has potential to be processed into useful products, for example through fermentation into garbage enzymes (GE) or also known as eco-enzymes. This study aims to determine 16S rRNA gene fragment amplicon of metagenomic DNA, antibacterial activity against the Staphylococcus aureus and Escherichia coli and cellulase activity from GE of sweet orange (Citrus sinensis) peels. Sweet orange peel was mixed with brown sugar and water in the ratio of 3:1:10 respectively, then fermented for 90 days (GE-90) and 105 days (GE-105). The metagenomic DNA isolate of GE was amplified by polymerase chain reaction (PCR) technique, antibacterial activity was tested using the agar diffusion well method, and cellulase activity was determined by the dinitrosalicylic acid (DNS) method. The results of GE-90 and GE-105 metagenomic DNA amplification using PCR was obtained 16S rRNA gene fragment of about 750 bp. Inhibition effectiveness of undiluted GE-90 against S. aureus and E. coli compared with amoxicillin 0.03% was 76.7 and 71.6%, respectively; while GE-105 was 68.7 and 56.6% respectively. Cellulase activity of GE-90 was 0.01186 ± 0.00077 U/mL; and 0.01384 ± 0.00113 U/mL for GE-105.
- Copyright
- © 2024 The Author(s)
- Open Access
- Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.
Cite this article
TY - CONF AU - I Nengah Wirajana AU - I Made Bramandita AU - I Made Sukadana PY - 2024 DA - 2024/05/30 TI - 16S rRNA Fragment of Metagenomic DNA, Antibacterial and Cellulase Activity from Garbage Enzymes of Sweet Orange (Citrus sinensis) Peels BT - Proceedings of selected papers of International Conference on Health, Science, and Environment (ICHSE 2023) PB - Atlantis Press SP - 163 EP - 171 SN - 2468-5747 UR - https://doi.org/10.2991/978-94-6463-431-0_17 DO - 10.2991/978-94-6463-431-0_17 ID - Wirajana2024 ER -