Biological Activity of Celery Extract Using Different Extraction Methods

DOI

10.2991/978-94-6463-112-8_30How to use a DOI?

Keywords

biological activity; celery; extraction; maceration; ultrasonic

Abstract

Celery is a medicinal plant that is widely used as an ingredient in traditional medicine in Indonesia. Scientifically, celery extract has the ability as an antioxidant, antibacterial, anti-breast cancer, and inhibiting ACE enzyme activity as antihypertensive. The use of maceration and ultrasound-assisted extraction (UAE) methods will give different results on the amount and biological activity of the extract. This study aims to determine the biological activity of celery extract using maceration and ultrasound-assisted extraction method. TEAC, FRAP, NO, and DPPH scavenging assays were used to determine in vitro antioxidant activities. Folin-Ciocalteu reagent and aluminium chloride colorimetric assay were used to determine total phenolic and flavonoid contents, respectively. The sulforhodamine B and angiotensin-converting enzyme (ACE) inhibition assays were used to determine anti-breast cancer and antihypertensive activities, respectively. The celery extract from both extraction methods had antioxidants activity, inhibited the growth of MDA-MB-231 breast cancer cell line, and showed as antihypertensive by ACE inhibition. The maceration gave better activities than UAE with significant results in antioxidants, especially in a water solvent. The use of solvents had more effect on antioxidant activity, especially the FRAP and DPPH free radical scavenging method, as well as the TPC and TFC values of the extract compared to the extraction technique. It was found that ethanol solvent was better than water. The maceration provided better activities than ultrasound-assisted extraction in anti-breast cancer. However, there was no significant difference in antihypertensives. The UAE extraction method produced higher yields but had lower antioxidant and anticancer activity than a maceration.

Copyright

© 2023 The Author(s)

Open Access

Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

Download article (PDF)

TY  - CONF
AU  - Eris Septiana
AU  - Siti I. Rahmawati
AU  - Fauzia N. Izzati
AU  - Peni Ahmadi
AU  - Diah A. Wulandari
AU  - Bustanussalam Bustanussalam
AU  - Mega F. Warsito
AU  - Masteria Y. Putra
PY  - 2023
DA  - 2023/03/01
TI  - Biological Activity of Celery Extract Using Different Extraction Methods
BT  - Proceedings of the 1st International Conference for Health Research – BRIN (ICHR 2022)
PB  - Atlantis Press
SP  - 312
EP  - 326
SN  - 2468-5739
UR  - https://doi.org/10.2991/978-94-6463-112-8_30
DO  - 10.2991/978-94-6463-112-8_30
ID  - Septiana2023
ER  -