Antioxidant and Antibacterial Properties Derived from Horsfieldia Spicata (Roxb.) J. Sinclair Stem Bark Extract and Its Active Fraction

DOI

10.2991/978-94-6463-112-8_31How to use a DOI?

Keywords

Antibacterial; Antioxidant; DPPH; Horsfieldia spicata; Staphylococcus aureus

Abstract

Horsfieldia spicata (Roxb.) J. Sinclair is a species of plant from the Myristicaceae family which has more than 100 species and is widely distributed in South Asia, from India to the Philippines and Papua New Guinea. Numerous species of this family are utilized for wood, and other species are reported to contain several groups of active compounds such as alkaloids, polyphenols, flavonoids and saponins. Various active compounds from this family are known to have various bioactivities including antioxidant, antibacterial, analgesic, and cytotoxic. This study was conducted to determine the value of antioxidant and antibacterial activities from the stem bark extract of H. spicata and its active fractions. Of note we used some bacterial tested of the gram negative, Pseudomonas aeruginosa (ATCC 9027) and Escherichia coli (FNCC-0195), as well as gram positive, namely Bacillus subtilis (FNCC-0059), and Staphylococcus aureus (FNCC-0047). The stems of H. spicata were extracted using methanol as a solvent to obtain a crude extract. Further, the extract was then fractionated to obtain n-hexane, ethyl acetate and butanol fractions. The extracts and its fractions were then tested for antioxidants using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) method. The sample was then assayed for antibacterial using the disc diffusion and Minimum Inhibitory Concentration (MIC) methods. The results of the antioxidant activity test showed that the ethyl acetate fraction had the strongest activity with an IC₅₀ value of 23.53 µg/mL, followed by the butanol fraction, methanol extract, and the n-hexane fraction with an IC₅₀ value of 63.36, 75.37 and 114.17 µg/mL, respectively. As for the antibacterial test results showed that the methanol extract had the best ability to inhibit the growth of B. subtilis and P. aeruginosa with clear zone values of 13.33 ± 2.36 and 15.33 ± 4.50 mm, respectively. On the other hand, the hexane fraction showed the best antibacterial activity against E. coli and S. aureus with clear zone values of 12.00 ± 1.41 and 12.33 ± 0.47 mm, respectively. MIC analysis showed that n-hexane fraction had the strongest activity value against P. aeruginosa, E. coli and B. subtilis with MIC value of 500, 125 and 500 µg/mL, respectively. These results indicate that the extract and active fractions from the stem bark of the H. spicata have the potential to be developed as an alternative raw material for pharmaceutical uses.

Copyright

© 2023 The Author(s)

Open Access

Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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TY  - CONF
AU  - Minarti Minarti
AU  - Novita Ariani
AU  - Muhammad E. Prastya
AU  - Akhmad Darmawan
AU  - Megawati Megawati
PY  - 2023
DA  - 2023/03/01
TI  - Antioxidant and Antibacterial Properties Derived from Horsfieldia Spicata (Roxb.) J. Sinclair Stem Bark Extract and Its Active Fraction
BT  - Proceedings of the 1st International Conference for Health Research – BRIN (ICHR 2022)
PB  - Atlantis Press
SP  - 327
EP  - 337
SN  - 2468-5739
UR  - https://doi.org/10.2991/978-94-6463-112-8_31
DO  - 10.2991/978-94-6463-112-8_31
ID  - Minarti2023
ER  -