Estimation of HSP70 in SiHa Cell Lines During Chemoradiation and Radiation

DOI

10.2991/978-94-6463-252-1_18How to use a DOI?

Keywords

Heat shock protein 70; cancer cell lines; Cervical cancer; chemotherapy; radiation

Abstract

Cervical cancer remains a significant health problem worldwide, despite the advances in screening, diagnosis, treatment, and prognosis. Heat shock protein 70 (HSP70) is an anti-apoptotic protein that inhibits the cancer cell death by interfering with apoptotic pathway activation. In chemoradiation, the production of the Reactive Oxygen Species damages the organelles of the cancer cells. This urges the dying cells to produce HSP70 which inhibits the extrinsic and intrinsic apoptotic pathways helping in the prognosis of cancer. In the present study before approaching human subjects, the hypothesis is tested on SiHa cervical squamous cell carcinoma cell line. The paper aims to estimate the anti-apoptotic HSP70 protein in the media collected from the cultured SiHa cervical squamous cell carcinoma cell line treated with cisplatin, radiation and chemoradation. The Hsp70 is estimated using ELISA method and the HSPA1A gene expression is estimated using qRT-PCR. From the radiation and chemoradiation results, the investigators noticed that cancer treatments induce cellular damage and initiate a cytoprotective mechanism in cells as a response. Higher levels of HSP70 are found in SiHa cells treated with cisplatin-combined radiation when compared to cisplatin or radiation alone. From the findings, the investigators conclude that the higher expression levels of HSP70 in chemoradiated SiHa cells showed that cytoprotective anti-apoptotic HSP70 protein is released as a response to chemoradiation.

Copyright

© 2023 The Author(s)

Open Access

Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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TY  - CONF
AU  - B. Sai Lalitha
AU  - Mudigonda Malini
AU  - M. Venkateswar Rao
AU  - Mahendran Botlagunta
PY  - 2023
DA  - 2023/11/09
TI  - Estimation of HSP70 in SiHa Cell Lines During Chemoradiation and Radiation
BT  - Proceedings of the Second International Conference on Emerging Trends in Engineering (ICETE 2023)
PB  - Atlantis Press
SP  - 149
EP  - 159
SN  - 2352-5401
UR  - https://doi.org/10.2991/978-94-6463-252-1_18
DO  - 10.2991/978-94-6463-252-1_18
ID  - SaiLalitha2023
ER  -