GH, GHRH, and PIT Genes Polymorphisms of Local Swamp Buffalo in Pandeglang District, Banten Province

DOI

10.2991/978-94-6463-116-6_8How to use a DOI?

Keywords

Swamp buffalo; growth family genes; PCR-RFLP; SNP

Abstract

The molecular selection of the genes influencing growth qualities can be used to genetically improve the production of native swamp buffaloes. Local swamp buffalo (60 hds.) maintained by smallholders in Pandeglang Regency, Banten Province, were examined for the genetic polymorphism of the growing family (GH, GHRH, and PIT1) genes. By using restriction enzymes of MspI (GH gene), HaeIII (GHRH gene), and HinfI (PIT-1 gene), the genotyping of GH g.1547T>C, GHRH g.4666G>C, and PIT-1g.1256G>A SNPs was found by polymerase chain reaction-restriction fragment polymorphism (PCR-RFLP). Genotyping on the respective GH_g.1547T>C, GHRH_g.4666G>C, and PIT-1_g.1256G>A loci, however, resulted in only one type of genotype i.e. CC, GG, and AA with one type of allele i.e. C, G, and A successively. Therefore, the frequencies were 100% or monomorphic for those genotypes and alleles of each growth gene. Also, the PIC values of each locus were 0.00, as were the heterozygosity observation (Ho) and expectation (He) values. It is possible to suggest increasing the genotype frequency of the SNPs of the relevant growth genes that are favorably correlated with the growth traits and associated economic traits of the observed local swamp buffalo in this area.

Copyright

© 2023 The Author(s)

Open Access

Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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TY  - CONF
AU  - A. Anggraeni
AU  - C. Thalib
AU  - F. Saputra
PY  - 2023
DA  - 2023/03/08
TI  - GH, GHRH, and PIT Genes Polymorphisms of Local Swamp Buffalo in Pandeglang District, Banten Province
BT  - Proceedings of the 3rd International Conference on Environmentally Sustainable Animal Industry 2022 (ICESAI 2022)
PB  - Atlantis Press
SP  - 50
EP  - 59
SN  - 2468-5747
UR  - https://doi.org/10.2991/978-94-6463-116-6_8
DO  - 10.2991/978-94-6463-116-6_8
ID  - Anggraeni2023
ER  -