New Collagen Scaffolds with Extra Cellular Matrix Derived from Treatments of Supercritical Fluid and Protease in Low Temperature
- DOI
- 10.2991/ice2me-19.2019.44How to use a DOI?
- Keywords
- ECM; enzymes; SCF
- Abstract
Nowadays, tissue-engineering was important and popular which combine medical applications and engineering materials knowledge, just like cell extraction process was employed to remove the cellular components from porcine dermal, leaving a framework of largely insoluble collagen, elastin, and tightly bound glycosaminoglycans (GAGs). A cell extraction process was used to remove cellular components by porcine dermal. Varying pore sizes and porosities of the acellular tissues were then created using enzymes and collagenase. The porcine dermal must remove fat and other impurities almost by supercritical fluid(SCF), otherwise adipose tissue will become rot immediately. For collagen scaffold samples, dermis were hydrolyzed by enzymes of different concentration, in this way it can gain state completely and clearly. Electron microscopy revealed effect in ECM of porcine dermal tissue via supercritical fluid and different concentrations enzymes. This suggests the enzymes concentration and when you put porcine dermal in SCF was mightily important for effect of the collagen.
- Copyright
- © 2019, the Authors. Published by Atlantis Press.
- Open Access
- This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).
Cite this article
TY - CONF AU - Ciao-Yi Syu AU - Yu-Lin Shen AU - Cong-Han Xiao AU - Tzu-Pei Lee AU - Meng-Jen Yang AU - Ing-Lin Chang AU - Ching-Cheng Huang PY - 2019/03 DA - 2019/03 TI - New Collagen Scaffolds with Extra Cellular Matrix Derived from Treatments of Supercritical Fluid and Protease in Low Temperature BT - Proceedings of the 2019 International Conference on Electronical, Mechanical and Materials Engineering (ICE2ME 2019) PB - Atlantis Press SP - 196 EP - 199 SN - 2352-5401 UR - https://doi.org/10.2991/ice2me-19.2019.44 DO - 10.2991/ice2me-19.2019.44 ID - Syu2019/03 ER -