Preparation of New Acellular Dermal Collagen Scaffolds via Combination of Phytoproteinases and Supercritical Carbon Dioxide Treatments
- DOI
- 10.2991/ice2me-19.2019.38How to use a DOI?
- Keywords
- ECM; enzymes; SCF
- Abstract
Tissue-engineering technology was important and popular which combine medical applications and engineering materials knowledge such as a cell extraction process, which was employed to remove the cellular components from porcine dermal, leaving a matrix of collagen, elastin, collagen, and tightly bound glycosaminoglycans(GAGs). Said cell extraction process was used to remove cellular components from porcine dermal materials. Different porosities and microstructure of the acellular tissues were could be created by using different concentrations of related enzymes and collagenase. In this study, the uniform porcine dermal would be obtained by using a designed machine. It get dermis exactly and quickly via a machine which was designed from our team(powerdriven dermatome, SIACIN international, SC-GST). The porcine dermal must remove fat and other impurities almost by supercritical fluid(SCF), otherwise adipose tissue will become rot immediately. For collagen scaffold samples, dermis were hydrolyzed by enzymes of different concentration, in this way it can gain stant completely and clearly. Electron microscopy revealed effect in extracellular matrix (ECM) of porcine dermal tissue via supercritical fluid and different concentrations enzymes. This suggests the enzymes concentration and when you put porcine dermal in SCF was mightily important for effect of the collagen.
- Copyright
- © 2019, the Authors. Published by Atlantis Press.
- Open Access
- This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).
Cite this article
TY - CONF AU - Wang-Yao Wu AU - Tse-wei Ciou AU - Kuan-Ta Chen AU - Tzu-hung Su AU - Meng-Jen Yang AU - Ing-Lin Chang AU - Ching-Cheng Huang PY - 2019/03 DA - 2019/03 TI - Preparation of New Acellular Dermal Collagen Scaffolds via Combination of Phytoproteinases and Supercritical Carbon Dioxide Treatments BT - Proceedings of the 2019 International Conference on Electronical, Mechanical and Materials Engineering (ICE2ME 2019) PB - Atlantis Press SP - 170 EP - 173 SN - 2352-5401 UR - https://doi.org/10.2991/ice2me-19.2019.38 DO - 10.2991/ice2me-19.2019.38 ID - Wu2019/03 ER -