Preliminary Study: Bioautography Screening on Edible Local Plants with α-Glucosidase Inhibitor
- DOI
- 10.2991/ahsr.k.211105.043How to use a DOI?
- Keywords
- diabetes mellitus type 2; TLC bioautography; rice; local vegetables
- Abstract
Indonesia is ranked as one of the top ten countries with higher diabetes mellitus cases in 2019. In total, International Diabetes Federation recorded 10.7 million diabetes mellitus cases in 2019. Many researchers reported that diabetes mellitus type 2 could be controlled and managed with diet. Since local food is made from common plants that people can easily find, we collected edible local plats from a small village in Solok Regency and screened their inhibition toward enzyme α-glucosidase. Fourteen local edible plants were collected for the screening of α-glucosidase inhibitors. The plants were macerated with methanol to gain methanolic extract. The extracts were spotted at a concentration of 10 mg/mL with a volume of 8 μL on a silica TLC plat and then eluted with semi-polar mobile phase, chloroform: ethyl acetate: methanol = 65:20:15. Afterward, the TLC plate was sprayed with α-glucosidase inhibitor. The results showed that two samples were inhibited by α-glucosidase. They were rice (Oryza sativa L.) and torch ginger (Etlingera elatior (Jack) R.M.Sm.). The active spots in rice had rf values at 0.66 and 0.81, while torch ginger had rf values at 0.23, 0.25, and 0.63.
- Copyright
- © 2021 The Authors. Published by Atlantis Press International B.V.
- Open Access
- This is an open access article under the CC BY-NC license.
Cite this article
TY - CONF AU - Nova Syafni AU - Nurwahidatul Arifa AU - Friardi Ismed AU - Deddi Prima Putra PY - 2021 DA - 2021/11/17 TI - Preliminary Study: Bioautography Screening on Edible Local Plants with α-Glucosidase Inhibitor BT - Proceedings of the 2nd International Conference on Contemporary Science and Clinical Pharmacy 2021 (ICCSCP 2021) PB - Atlantis Press SP - 295 EP - 301 SN - 2468-5739 UR - https://doi.org/10.2991/ahsr.k.211105.043 DO - 10.2991/ahsr.k.211105.043 ID - Syafni2021 ER -