In Vivo Cytotoxic Activity and Acute Toxicity Test of Ethanol Extract from Voacanga foetida (Blume) Rolfe Leaves
- DOI
- 10.2991/ahsr.k.211105.003How to use a DOI?
- Keywords
- Voacanga foetida; cytotoxic; toxicity
- Abstract
In vivo cytotoxic activity and acute toxicity test of ethanolic extract Voacanga foetida leave against male white mice was done. The in vivo cytotoxic activity used Micronucleus Assay Method while the acute toxicity used LD50 Test and Delay Toxicity Method. The micronucleus assay of V. foetida ethanol extract on the concentration of 100, 200, and 300 mg/kg BW with the observation of the amount of micronuclei cell of femur bone marrow of mice. The micronucleus assay on dose 200 mg/kg BW of the ethanol extract from V. foetida leaves was potential as anticarcinogenic activity. The LD50 of V. foetida ethanol extract for 24 hours of the animal was observed on the dose of 800, 1600, 3200, and 6400 mg/kg BW. The delayed toxicity test results, for the dietary intake, fecal, urine volume, organ weight ratio, and pathophysiologic of the spleen, heart, and liver through the qualitative and quantitatively against that organs. The results showed that the ethanolic extract of V. foetida was classified as not toxic with LD50 for 24 h > 15,000 mg/kg BW. The delayed toxicity test of V. foetida showed that this extract could decrease the daily dietary intake, water consumption, urine volume, fecal, body weight, and organ weight for 14 days of observation. The histopathologic test from dose 1600 mg/kg BW of V.foetida extract might be toxic against the heart and kidneys, but not toxic to the liver.
- Copyright
- © 2021 The Authors. Published by Atlantis Press International B.V.
- Open Access
- This is an open access article under the CC BY-NC license.
Cite this article
TY - CONF AU - Adriani Susanty AU - Emrizal AU - Emma Susanti AU - Alfionita BR Saragih AU - Husni Muchtar AU - Dachriyanus PY - 2021 DA - 2021/11/17 TI - In Vivo Cytotoxic Activity and Acute Toxicity Test of Ethanol Extract from Voacanga foetida (Blume) Rolfe Leaves BT - Proceedings of the 2nd International Conference on Contemporary Science and Clinical Pharmacy 2021 (ICCSCP 2021) PB - Atlantis Press SP - 14 EP - 20 SN - 2468-5739 UR - https://doi.org/10.2991/ahsr.k.211105.003 DO - 10.2991/ahsr.k.211105.003 ID - Susanty2021 ER -