Molecular Confirmation of Forensic Important Flies Collected in Palembang, South Sumatra

DOI

10.2991/absr.k.200513.038How to use a DOI?

Keywords

Calliphoridae, Sarcophagidae, cytochrome C oxidase subunit II, morphology

Abstract

The identification of insect species is an important key in forensic entomology. Molecular examination of the nucleotide sequences of cytochrome c oxidase subunit II (COII) gene has been widely performed before the morphology identification. We analyzed the partial conserved COII nucleotide sequence of the forensic related flies. After the DNA extraction from collected larvae, thus PCR amplification of conserved sequencing of the partial COII sequence was implemented. Obtained sequences were analyzed for a phylogenetic tree and a distance matrix. Our data showed a very low intraspecific sequence distance among Calliphoridae and Sarcophagidae to the flies’ species of Chrysomyia megacephala. Further research should attempt to determine the larval growth of newly discovered flies, to apply the time of death in forensic crime setting.

Copyright

© 2020, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Ahmad Ghiffari
AU  - Chairil Anwar
AU  - Dalilah
AU  - Mansuri
AU  - Elfinchia Tiara Switha
PY  - 2020
DA  - 2020/05/18
TI  - Molecular Confirmation of Forensic Important Flies Collected in Palembang, South Sumatra
BT  - Proceedings of the International Conference and the 10th Congress of the Entomological Society of Indonesia (ICCESI 2019)
PB  - Atlantis Press
SP  - 219
EP  - 224
SN  - 2468-5747
UR  - https://doi.org/10.2991/absr.k.200513.038
DO  - 10.2991/absr.k.200513.038
ID  - Ghiffari2020
ER  -