Monitoring Lymphatic Filariasis Interventions Through Adult Mosquito PCR Sampling in South Sumatra Province, Indonesia

DOI

10.2991/absr.k.200513.039How to use a DOI?

Keywords

elephantiasis, Brugia malayi, Culex quinquefasciatus, HLC gene, surveillance

Abstract

Monitoring and evaluation are essential to the successful implementation of mass drug administration programs for lymphatic filariasis elimination. PCR-based methods are preferred over classical dissections with the protocol of one L3 Brugia malayi larva in a pool of mosquitoes. We analysed the partial HCL gene nucleotide sequence of the parasite inside the vector. Samples were collected with the methods of human landing, pyrethrum spray, and light traps catch, conducted concurrently at sites in an LF endemic Banyuasin district in South Sumatra. The DNA extraction method was DNAeasy Tissue kit (Quaigen Inc) were used on pools of 10 mosquitoes each. These were used as template DNA in PCR to amplify B. malayi sequences and thus sequenced. Our data showed a sequence of the filarial worm in Mansonia annulifera and Culex quinquefasciatus. To the best of our knowledge, this study is the first report of HLC gene nucleotide sequences from the species Cx. quinquefasciatus.

Copyright

© 2020, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Ahmad Ghiffari
AU  - Rini Pratiwi
AU  - Chairil Anwar
AU  - Dalilah
PY  - 2020
DA  - 2020/05/18
TI  - Monitoring Lymphatic Filariasis Interventions Through Adult Mosquito PCR Sampling in South Sumatra Province, Indonesia
BT  - Proceedings of the International Conference and the 10th Congress of the Entomological Society of Indonesia (ICCESI 2019)
PB  - Atlantis Press
SP  - 225
EP  - 228
SN  - 2468-5747
UR  - https://doi.org/10.2991/absr.k.200513.039
DO  - 10.2991/absr.k.200513.039
ID  - Ghiffari2020
ER  -