Proceedings of the 7th International Conference on Biological Science (ICBS 2021)

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Developing RAPD-derived SCAR (Sequence Characterized Amplified Region) Marker for Flowering Time in Chili Pepper

Authors
Estri Laras Arumingtyas1, *, Bunga Rizky Elfa Agustina2, Joni Kusnadi2
1Biology Department, Faculty of Mathematics and Natural Sciences, Brawijaya University
2Department of Agricultural Product Processing, Faculty of Agricultural Technology, Brawijaya University
*Corresponding author. Email: larasbio@gmail.com
Corresponding Author
Estri Laras Arumingtyas
Available Online 2 May 2022.
DOI
10.2991/absr.k.220406.012How to use a DOI?
Keywords
Chili Pepper; Flowering; RAPD; SCAR
Abstract

The selection step of plant breeding is a crucial stage in the process of developing new varieties which usually take a considerable long time in a conventional breeding. The development of molecular markers for selection has been carried out to reduce the time required. A Sequence Characterized Amplified Region (SCAR) molecular marker based on the Random Amplified Polymorphic DNA (RAPD) DNA profile was developed to detect fast-flowering strains in chili pepper. From the RAPD stage which was amplified using 20 RAPD primers, 1 primer (OPB6) was obtained which produced polymorphic DNA bands that could distinguish between Early-Flowering and Late-Flowering lines. A polymorphic band sequence measuring approximately 600 bp was used as the basis for the SCAR Upper F primer design: 5’-GACAACC CCACACTACA TAC-3’; R: 5’-AACT GAACGAGGAA GCGTAG-3’, Lower 1 F: 5’-GGAT GGAGTGAGAG AACCTA-3’; R: 5’-GAGA AAGTTCTAGG GCAGAG-3’ and Lower 2. F: 5’-GAAC CTCCAACCTC ACGTTT-3’; R: 5’-GAA GAGGTGATGA TGTCAGC-3’. The effectivity test for the SCAR primers did not provide specific and accurate result. Further refinement needs to be done to obtain more specificity.

Copyright
© 2022 The Authors. Published by Atlantis Press International B.V.
Open Access
This is an open access article distributed under the CC BY-NC 4.0 license.

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Volume Title
Proceedings of the 7th International Conference on Biological Science (ICBS 2021)
Series
Advances in Biological Sciences Research
Publication Date
2 May 2022
ISBN
978-94-6239-573-2
ISSN
2468-5747
DOI
10.2991/absr.k.220406.012How to use a DOI?
Copyright
© 2022 The Authors. Published by Atlantis Press International B.V.
Open Access
This is an open access article distributed under the CC BY-NC 4.0 license.

Cite this article

risenwbib
TY  - CONF
AU  - Estri Laras Arumingtyas
AU  - Bunga Rizky Elfa Agustina
AU  - Joni Kusnadi
PY  - 2022
DA  - 2022/05/02
TI  - Developing RAPD-derived SCAR (Sequence Characterized Amplified Region) Marker for Flowering Time in Chili Pepper
BT  - Proceedings of the 7th International Conference on Biological Science (ICBS 2021)
PB  - Atlantis Press
SP  - 78
EP  - 81
SN  - 2468-5747
UR  - https://doi.org/10.2991/absr.k.220406.012
DO  - 10.2991/absr.k.220406.012
ID  - Arumingtyas2022
ER  -