UV Protectant Ability of Attacus atlas L. (Lepidoptera: Saturniidae) Sericin Extract to Increase Nucleopolyhedrovirus Effectiveness against Beet Army Worm, Spodoptera exigua (Hübner) (Lepidoptera: Noctuidae)

DOI

10.2991/absr.k.220406.013How to use a DOI?

Keywords

Attacus atlas; Nucleopolyhedrovirus; Spodoptera exigua; Ultraviolet

Abstract

Spodoptera exigua (Lepidoptera: Noctuidae) is the common pest known for attacking shallot crop. Baculovirus (Nucleopolyhedrovirus: NPV) is a biological agent that is widely used as the pest control agent. However, the activity of NPV is deteriorated when applied in the field due to the influence of ultraviolet from the sun. Attacus atlas silkworm cocoon has UV protectant ability due to the presence of sericin protein. Thus, the aim of this study is to determine the degree of UV protectant ability from A. atlas cocoon extract for NPV to prevent its deterioration and to increase its effectiveness against S. exigua pest. The stages of this study consist of UVB irradiation of A. atlas cocoon extract as NPV protectant and pathogenicity test of NPV activity against the first instar larvae of S. exigua. Experiments were carried out by exposing NPV to UVB irradiation for 0, 1, 2, 3, 4 weeks long with the addition of A. atlas cocoon extract in varied concentrations (0, 0.5, 1, 2, and 2.5%). The concentrations derived from 7.5 gram A. atlas cocoon was dilluted 1 gram TRO and 150 ml aquades. The results proved the UV protectant ability of A. atlas cocoon for NPV. After a week of UVB irradiation, the NPV applied in A. atlas cocoon extract (0-2.5%) caused larval mortality of 43.33%, 76.67%, 60%, 65%, 80%, respectively. After 2 weeks of UVB irradiation, the NPV applied in A. atlas cocoon extract (0-2.5%) caused larval mortality of 46.67%, 78.33%, 71.67%, 66.67%, 66.67%, respectively. Thus, for further applications, the findings of this research need to be tested in an actual field condition.

Copyright

© 2022 The Authors. Published by Atlantis Press International B.V.

Open Access

This is an open access article distributed under the CC BY-NC 4.0 license.

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TY  - CONF
AU  - Hana Widiawati
AU  - Sukirno Sukirno
AU  - Siti Sumarmi
AU  - Hari Purwanto
AU  - R.C. Hidayat Soesilohadi
AU  - Ignatius Sudaryadi
PY  - 2022
DA  - 2022/05/02
TI  - UV Protectant Ability of Attacus atlas L. (Lepidoptera: Saturniidae) Sericin Extract to Increase Nucleopolyhedrovirus Effectiveness against Beet Army Worm, Spodoptera exigua (Hübner) (Lepidoptera: Noctuidae)
BT  - Proceedings of the 7th International Conference on Biological Science (ICBS 2021)
PB  - Atlantis Press
SP  - 82
EP  - 89
SN  - 2468-5747
UR  - https://doi.org/10.2991/absr.k.220406.013
DO  - 10.2991/absr.k.220406.013
ID  - Widiawati2022
ER  -