Near-infrared Fluorescence Imaging and Optimization of Polyacrylamide

DOI

10.2991/icammce-18.2018.97How to use a DOI?

Keywords

Polyacrylamide, Fluorescence, Effect Factors

Abstract

Polyacrylamide (PAA) is a reticular macromolecular compound, and the C=N bonds produced by the reaction with glutaraldehyde reveal intensive near-infrared fluorescence (NIRF). This paper studies the effect factors on the fluorescence intensity of PAA microspheres (beads), including the particle size of PAA beads, APMA concentrations, and different concentrations of glycine treatment by infrared imaging system and fluorescence microscope. The results demonstrate that PAA gel and PAA beads display autofluorescence at the emission wavelength of 700 nm and 800 nm，respectively. The smaller the particle size of beads is, the better the fluorescence intensity is in a certain range. The beads have stronger NIRF when 11 mg/mL APMA is in monomer liquid compared with the beads containing 7 mg/mL or 9 mg/mL APMA in monomer liquid. Glycine treatment enhances the fluorescence intensity of the beads, which is the most obvious at 0.2 M glycine in solution.

Copyright

© 2018, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Chen Yan
AU  - Jinke Wang
PY  - 2018/05
DA  - 2018/05
TI  - Near-infrared Fluorescence Imaging and Optimization of Polyacrylamide
BT  - Proceedings of the 2018 3rd International Conference on Advances in Materials, Mechatronics and Civil Engineering (ICAMMCE 2018)
PB  - Atlantis Press
SP  - 454
EP  - 457
SN  - 2352-5401
UR  - https://doi.org/10.2991/icammce-18.2018.97
DO  - 10.2991/icammce-18.2018.97
ID  - Yan2018/05
ER  -