Preparation of Autofluorescent Polyacrylamide Nanoparticles

DOI

10.2991/icammce-18.2018.96How to use a DOI?

Keywords

Polyacrylamide Nanoparticles, Glutaraldehyde, Near infrared, Fluorescence

Abstract

Nowadays, tumor imaging is widely used in biomedical field. However, the materials of imaging is rarely and strict. This study developed a method for preparing autofluorescent polyacrylamide nanoparticles (NPs). The polyacrylamide NPs were treated with glutaraldehyde, and then removed excess glutaraldehyde through ultrafiltration, glycine neutralization and ethanol precipitation, respectively. Based on this method, the polyacrylamide NPs showed strong near-infrared fluorescence (NIRF) at the emission wavelength of both 720 and 820 nm. Additionally, the nanoparticles were efficiently internalized into RAW264.7 cells and displayed strong green and red fluorescence observed with fluorescence microscope. Therefore, the NPs are expected to be applied to tumor imaging in future.

Copyright

© 2018, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Qiao Wang
AU  - Jinke Wang
PY  - 2018/05
DA  - 2018/05
TI  - Preparation of Autofluorescent Polyacrylamide Nanoparticles
BT  - Proceedings of the 2018 3rd International Conference on Advances in Materials, Mechatronics and Civil Engineering (ICAMMCE 2018)
PB  - Atlantis Press
SP  - 450
EP  - 453
SN  - 2352-5401
UR  - https://doi.org/10.2991/icammce-18.2018.96
DO  - 10.2991/icammce-18.2018.96
ID  - Wang2018/05
ER  -