Proceedings of the 3rd International Conference on Material, Mechanical and Manufacturing Engineering

Determination of Aflatoxin contaminants in chrysanthemum by HPLC

Authors
Yubing Zha, Yueping Su, Chunliang G, Yingqiang Huang, Xiaofang Wang, Ling Lin, Shaodong Zeng
Corresponding Author
Yubing Zha
Available Online August 2015.
DOI
10.2991/ic3me-15.2015.92How to use a DOI?
Keywords
HPLC; chrysanthemum; Aflatoxins;
Abstract

A method was developed for the simultaneous determination of Afiatoxin ( AFB1 AFB2 AFG1 AFG2) in chrysanthemum is developed by Liquid chromatograPhy with fluoreseence detection after immunoaffinity column clean up and Pre cloumn derivatization.four south medicine samples were extracted with methanol water(70:30,v/v),The extracts were Purified by imunoaffinity columns and the toxins were separated by reversed phase HPLC, and quantified with fluorescenee detection after photoehemieal derivatization. The separation was perfored on a C18 column.Recoveries of the different medicines spiked with Aflatoxin, The average recoveries ranged from 81.0 % 94.3 % by addition of avermectin standards at three different concentration levels of 10 g/kg) to chrysanthemum as matrixes. The sensitivity, accuracy and precisiothod were able to meet the requirements for pesticide residue analysis.

Copyright
© 2015, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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Volume Title
Proceedings of the 3rd International Conference on Material, Mechanical and Manufacturing Engineering
Series
Advances in Engineering Research
Publication Date
August 2015
ISBN
978-94-6252-100-1
ISSN
2352-5401
DOI
10.2991/ic3me-15.2015.92How to use a DOI?
Copyright
© 2015, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

Cite this article

TY  - CONF
AU  - Yubing Zha
AU  - Yueping Su
AU  - Chunliang G
AU  - Yingqiang Huang
AU  - Xiaofang Wang
AU  - Ling Lin
AU  - Shaodong Zeng
PY  - 2015/08
DA  - 2015/08
TI  - Determination of Aflatoxin contaminants in chrysanthemum by HPLC
BT  - Proceedings of the 3rd International Conference on Material, Mechanical and Manufacturing Engineering
PB  - Atlantis Press
SP  - 466
EP  - 468
SN  - 2352-5401
UR  - https://doi.org/10.2991/ic3me-15.2015.92
DO  - 10.2991/ic3me-15.2015.92
ID  - Zha2015/08
ER  -