Evaluation of in vitro Growth and Development of Banana cv. Barangan Plantlets as Affected by Biotin Application
- DOI
- 10.2991/978-94-6463-510-2_2How to use a DOI?
- Keywords
- Plant Micropropagation; Plantain; Musa sp.; Growth Substances; Vitamin
- Abstract
The presence of vitamins in culture medium has proven to be important during tissue culture of variuous species, including banana. Although there are many literatures on banana tissue culture, specific studies on banana cv. Barangan are limited. Through this study we investigate the effect of Biotin as a source of vitamins on plantlet regeneration through tissue culture. The study was conducted at the Plant Biotechnology Laboratory, University of Jambi. Plant material used were slices of banana cv. Barangan sucker cultured on MS basal medium. Biotin was tested at 0.0, 0.5 and 2.5 mg·L-1. The trial was arranged in a completely randomized design with 10 replications, in which each experimental unit consisted of 4 cultures. Variables observed were callus proliferation (proliferating time, color, structure, and texture), shoot formation (time of emergence of shoots, number of shoots and length of shoots), and root formation (time of emergence of roots, number of roots and length of the longest roots). Data collected during the study were analyzed using Analysis of Variance followed by the Least Significant Difference test at 5% confidence interval to see the difference in effect between treatment means. The results of the study showed that, the rate of callus proliferation on the explants of banana cv. Barangan could be enhanced by the addition of Biotin to the culture medium. In addition, Biotin application up to 2.5 mL-1 was proven to be effective to promote the growth and development of banana cv. Barangan plantlets in tissue culture system.
- Copyright
- © 2024 The Author(s)
- Open Access
- Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.
Cite this article
TY - CONF AU - Zulkarnain Zulkarnain AU - Eliyanti Eliyanti AU - Budiyati Ichwan PY - 2024 DA - 2024/09/16 TI - Evaluation of in vitro Growth and Development of Banana cv. Barangan Plantlets as Affected by Biotin Application BT - Proceedings of the International Agriculture Meeting (IAM 2023) PB - Atlantis Press SP - 4 EP - 14 SN - 2468-5747 UR - https://doi.org/10.2991/978-94-6463-510-2_2 DO - 10.2991/978-94-6463-510-2_2 ID - Zulkarnain2024 ER -