Propolis Trigona sp. Mouthwash Efectiveness in Lowering Anaerobic Gram-Negative Bacteria Colonies

DOI

10.2991/hsic-17.2017.29How to use a DOI?

Keywords

Propolis Trigona Sp.; Mouthwash; Anarobic Gram-Negative Bacteria

Abstract

Periodontal disease is an infectious disease that attacks gingiva and other dental supporting tissue which caused by microbial bacteria in subgingival plaque. The majority bacteria are an anaerobic gram-negative. Propolis is one of natural products as an antimicrobial mouthwash. One type of bee that able to produce propolis in large quantities is Trigona sp. which is common in South Sulawesi. Objective: The aim of this research is to know the effectiveness of propolis Trigona sp. as a mouthwash against some colonies of anaerobic gram-negative bacteria. Method: The type of this research is used pretest-posttest with control group design. A sample size of 28 people was selected with the research criteria who visited the Periodontology Department of Dental Hospital of Hasanuddin University. The treatment group rinse using extract propolis 5% and 10% and aquades as a control. Negative anarobic bacterial carried out from sulcus gingival fluid, then cultivated in medium MacConkey using spread method. Statistical analyzes used were T paired, and Anova test (p<0,05). Result: Paired T-test results showed that there was a difference of the number of anaerobic gram-negative bacteria colonies between before and after 14 days of treatment in the propolis group of 5% and 10% concentration (p = 0,000), when in the control group (aquades) there was no difference (p = 0.057). Anova test results showed that there was a decrease in the number of anaerobic gram-negative bacteria colonies between 5%, 10% propolis and control group (p = 0,000). Conclusion: Extract propolis Trigona sp. 5% and 10% which is contained in mouthwash is effective in lowering the number of anaerobic gram-negative bacteria colonies. The use of mouthwash with extractives of propolis may be recommended as an alternative therapy for periodontal disease.

Copyright

© 2017, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Rasmidar Samad
AU  - Fuad Husain Akbar
AU  - Nursyamsi Nursyamsi
AU  - Melinda Awing
PY  - 2017/10
DA  - 2017/10
TI  - Propolis Trigona sp. Mouthwash Efectiveness in Lowering Anaerobic Gram-Negative Bacteria Colonies
BT  - Proceedings of the Health Science International Conference (HSIC 2017)
PB  - Atlantis Press
SP  - 181
EP  - 187
SN  - 2468-5739
UR  - https://doi.org/10.2991/hsic-17.2017.29
DO  - 10.2991/hsic-17.2017.29
ID  - Samad2017/10
ER  -