The Study of Relationship Between the Number of Protozoa and Inoculum pH on the in vitro Technique Incubating Treated Colleus amboinicus

DOI

10.2991/aer.k.210825.017How to use a DOI?

Keywords

Protozoa, pH, Colleus Amboinicus, Inoculum, Relationship

Abstract

This experiment aimed to reveal the relationship between the number of protozoa and the pH of inoculum in incubating different treated Colleus amboinicus in vitro technique. A sample of dry Colleus amboinicus was powdered and then treated with four treatments. There were four treatments including Colleus amboinicus without treatment as control, Colleus amboinicus put into a capsule, Colleus amboinicus treated with tannin, and Colleus amboinicus treated with saponin for T0, T1, T2, and T3 respectively. Treated Colleus amboinicus was incubated following in vitro gas technique. At the end of the incubation period, inoculum pH was measured and around 3 ml of samples was used for calculating protozoa number. Data were statistically analyzed for regression and correlation. The result of this experiment shows that there was a low negative correlation between protozoa number and inoculum pH (r2 = -0.1999) but the effect of protozoa number on the inoculum pH was also negative on the inocullum pH.

Copyright

© 2021, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Muhammad Afdal
AU  - Darlis
AU  - Adriani
PY  - 2021
DA  - 2021/08/26
TI  - The Study of Relationship Between the Number of Protozoa and Inoculum pH on the in vitro Technique Incubating Treated Colleus amboinicus
BT  - Proceedings of the 3rd Green Development International Conference (GDIC 2020)
PB  - Atlantis Press
SP  - 87
EP  - 90
SN  - 2352-5401
UR  - https://doi.org/10.2991/aer.k.210825.017
DO  - 10.2991/aer.k.210825.017
ID  - Afdal2021
ER  -