Production of Biodiesel From Unrefined Plants-Derived Oil by Aspergillus oryzae Expressing Bacillus thermocatenulatus Lipase as a Whole-Cell Biocatalyst
- DOI
- 10.2991/ahe.k.210205.060How to use a DOI?
- Keywords
- biodiesel, Bacillus thermocatenulatus lipase, crude palm oil, enzymatic transesterification
- Abstract
In this study, the enzymatic process reaction using a whole-cell biocatalyst was done for biodiesel production of crude plant oils. This work investigated a streamlined technique to convert crude palm oil that consisted high amount of phosphocholine gums. This reaction by using lipase as a catalyst for biodiesel-fuel has an excellent potential compared with chemical reactions. We explored Aspergillus oryzae expressing Bacillus thermocatenulatus lipase (BTL) as a biocatalyst in producing fatty acid methyl FAME (95.54%) obtained at 30 oC, with 5.00 % (w/w) of BTL at 96 h using four molar equivalents of methanol. Therefore, BTL could be useful as whole-cell biocatalyst that can produce biodiesel from feedstock with a high proportion of impurities in industrial application.
- Copyright
- © 2021, the Authors. Published by Atlantis Press.
- Open Access
- This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).
Cite this article
TY - CONF AU - Nova Rachmadona AU - Martha Aznury AU - Chiaki Ogino PY - 2021 DA - 2021/02/09 TI - Production of Biodiesel From Unrefined Plants-Derived Oil by Aspergillus oryzae Expressing Bacillus thermocatenulatus Lipase as a Whole-Cell Biocatalyst BT - Proceedings of the 4th Forum in Research, Science, and Technology (FIRST-T1-T2-2020) PB - Atlantis Press SP - 358 EP - 360 SN - 2589-4943 UR - https://doi.org/10.2991/ahe.k.210205.060 DO - 10.2991/ahe.k.210205.060 ID - Rachmadona2021 ER -