Proceedings of the 5th International Conference on Food, Agriculture and Natural Resources (FANRes 2019)

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Endophytic Mycoflora from Mace and Seed of Nutmeg (Myristica fragrans Houtt) as a Source of Antifungal Compounds

Authors
Nurhasanah, Muhammad Rafly, Nurmaya Papuangan
Corresponding Author
Nurhasanah
Available Online 30 March 2020.
DOI
10.2991/aer.k.200325.075How to use a DOI?
Keywords
antifungal activity, endophytic fungi, mace and seed, nutmeg
Abstract

The nutmeg extract had been recognized as the source of antioxidant and antimicrobial activity. The aims of this study were to obtain endophytic fungi isolated from the mace and seed of Nutmeg (Myristica fragrans Houtt) and to determine antifungal activity of those isolates. Microbiol routine assay were conducted and it consists of three stages, namely isolation of endophytic fungi, identification of fungus and antifungal activity test by using dual culture. Sixteen endophytic fungus isolates were found in this study. Those are identified as Alternaria sp (BPB 1.3), Aspergilus sp (BPB 2.2, BPB 2.3, BPB 2.4) and sterile hyphae (FPB 1.1, FPB 2.2, FPB 3.3, FPB 3.4, FPB 4.3, FPB 5.2, FPB 5.3, BPB 3.1, BPB 3.2, BPB 5.1, BPB 5.2, and BPB 5.4), respectively. The isolate from Mace organ (FPB 2.2) had the highest antifungal activity, 51.67% while FPB 1.1 isolate had the lowest one (5%). The BPB 3.2 isolate had the highest antifungal activity in seed organ while BPB 5.2 isolate had the lowest ones, respectively 63,48% and 12,5%. Endophytic fungi which isolated from mace and seed of nutmeg (M. fragrans Houtt) are the potential to be used as an antifungal.

Copyright
© 2020, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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Volume Title
Proceedings of the 5th International Conference on Food, Agriculture and Natural Resources (FANRes 2019)
Series
Advances in Engineering Research
Publication Date
30 March 2020
ISBN
978-94-6252-942-7
ISSN
2352-5401
DOI
10.2991/aer.k.200325.075How to use a DOI?
Copyright
© 2020, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

Cite this article

risenwbib
TY  - CONF
AU  - Nurhasanah
AU  - Muhammad Rafly
AU  - Nurmaya Papuangan
PY  - 2020
DA  - 2020/03/30
TI  - Endophytic Mycoflora from Mace and Seed of Nutmeg (Myristica fragrans Houtt) as a Source of Antifungal Compounds
BT  - Proceedings of the 5th International Conference on Food, Agriculture and Natural Resources (FANRes 2019)
PB  - Atlantis Press
SP  - 381
EP  - 384
SN  - 2352-5401
UR  - https://doi.org/10.2991/aer.k.200325.075
DO  - 10.2991/aer.k.200325.075
ID  - 2020
ER  -