Anti-tumor Effects Elicited by Heat Shock Protein 72 Functional Peptide and Alpha-fetoprotein Epitope Peptide Vaccine

DOI

10.2991/emcs-17.2017.19How to use a DOI?

Keywords

Heat shock protien 72 (HSP72); Alpha-fetoprotein (AFP); Peptide; Tumor; Vaccine

Abstract

Objective: Alpha-fetoprotein (AFP) is an oncofetal antigen during hepatocellular carcinoma (HCC) development which could lead to weak reproducible antitumor immunity, and may act as a target for cancer therapy. Therefore, it is imperative to enhance its immunogenicity and develop therapeutic vaccines to eliminate AFP-expressing tumors. The study is intended to develop a specific anti-tumor peptide vaccine-heat shock protien 72 functional peptide (HSP72-P) and alpha-fetoprotein epitope peptide (AFP-P). Methods: By way of glutaraldehyde cross-linking, we constructed a potential therapeutic peptide vaccine, HSP72-P/AFP-P. In vitro and in vivo cytotoxicity assays were uesd to verify anti-tumor effect of the recombined vaccine. Results: The results showed that cross-linking of AFP epitope peptide with HSP72 functional peptide induced significant enhancement in specific AFP CD8+ T cells response and distinct cytotoxic antitumor reaction against AFP-expressing tumors. Primimg mice with the reconstructed vaccine, we elicited robust strong protective immunity. Conclusion: Our study suggests that tumor vaccination through cross-linking tumor antigen peptide and HSP72 functional peptide is an encouraging method for cancer immunotherapy.

Copyright

© 2017, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Xiaoping Wang
AU  - Qiaoxia Wang
AU  - Bing Xu
AU  - Na Chang
PY  - 2017/03
DA  - 2017/03
TI  - Anti-tumor Effects Elicited by Heat Shock Protein 72 Functional Peptide and Alpha-fetoprotein Epitope Peptide Vaccine
BT  - Proceedings of the 2017 7th International Conference on Education, Management, Computer and Society (EMCS 2017)
PB  - Atlantis Press
SP  - 95
EP  - 100
SN  - 2352-538X
UR  - https://doi.org/10.2991/emcs-17.2017.19
DO  - 10.2991/emcs-17.2017.19
ID  - Wang2017/03
ER  -