Effect of Nitric Oxide on Endothelial Differentiation of Mesenchymal Stem Cells

DOI

10.2991/cmfe-15.2015.36How to use a DOI?

Keywords

mesenchymal stem cells; Nitric oxide; differentiation; endothelial cells; RT-PCR

Abstract

Nitric oxide (NO), as a signaling molecule in vivo, plays an important regulatory role in a variety of physiological and pathological conditions. Mesenchymal stem cells (MSCs) are pluripotent progenitors that can differentiate into a variety of cell types. Vascular endothelial growth factor (VEGF) induces MSC differentiation towards endothelial cells (ECs). Although nitric oxide signaling promotes differentiation and maturation of endothelial progenitor cells, its role in endothelial differentiation of mesenchymal stem cells (MSCs) remains controversial. Frist, cellular proliferation was tested in MSCs treated with/without NO donors S-Nitrosoglutathione (GSNO) or Sodium Nitroprusside (SNP) by MTT assay. Next, after MSCs were cultured with different concentrations of NO donors in the presence of VEGF for 7 days, the cellular morphology was observed under phase microscope and the level of endothelial cell markers fms-like tyrosine kinase (Flt-1) was detected by RT-PCR and immunocytochemistry. The results determined that NO donors (1-200?mol/mL) had no effect on the survival of MSCs. VEGF-treated MSCs exhibited an endothelial-like phenotype, while NO donors-treated cells still have a spindle-shaped phenotype like MSCs. VEGF treatment increased Flt-1 expression, whereas NO donors inhibited VEGF-induced Flt-1 expression. Our Results suggested that NO could inhibit endothelial differentiation of mesenchymal stem cells.

Copyright

© 2015, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Nan Wang
AU  - Li-Nan Zhang
AU  - Rui Zhang
AU  - Na Huang
AU  - Zhi-Xia Guo
AU  - Tong-Cun Zhang
PY  - 2015/07
DA  - 2015/07
TI  - Effect of Nitric Oxide on Endothelial Differentiation of Mesenchymal Stem Cells
BT  - Proceedings of the International Conference on Chemical, Material and Food Engineering
PB  - Atlantis Press
SP  - 150
EP  - 153
SN  - 2352-5401
UR  - https://doi.org/10.2991/cmfe-15.2015.36
DO  - 10.2991/cmfe-15.2015.36
ID  - Wang2015/07
ER  -