Enzymatic Properties of Phytase from Escherichia coli DH5α

DOI

10.2991/cmfe-15.2015.22How to use a DOI?

Keywords

Escherichia coli; Phytase; Enzymatic properties; Molybdenum blue method; Enzyme activity

Abstract

A Escherichia coli strain producing phytase named DH5α was chosen from four E. coli stains, the enzyme activity was 17.88 U/mL after fermented at 37°C for 2 days. The enzymatic properties of crude enzyme solution were studied. The optimal temperature was 55°C; optimal pH was 2.5 and 7.0, enzyme had certain resistance to heat and different pH conditions, 75% activity could be maintained when treated at 65°C for 30min, and 60% activity still remained when treated in the pH range of 2 to 9 for 30 min and 60 min. Resistance to KH2PO4 of phytase was poor, low concentrations of KH2PO4 (15mg/L) could inhibit the enzyme activity to below 40%; all kinds of metal ions had no significant activation or inhibition effect on enzyme, the enzyme had resistance to pepsin but no resistance to trypsin, after treated by trypsin for 30min, only 66.69% of the activity retained, while treated by pepsin , the activity could still maintain more than 90%.

Copyright

© 2015, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Tao Wang
AU  - Wen Li
AU  - Yao Hao
AU  - Yang Liu
PY  - 2015/07
DA  - 2015/07
TI  - Enzymatic Properties of Phytase from Escherichia coli DH5α
BT  - Proceedings of the International Conference on Chemical, Material and Food Engineering
PB  - Atlantis Press
SP  - 88
EP  - 92
SN  - 2352-5401
UR  - https://doi.org/10.2991/cmfe-15.2015.22
DO  - 10.2991/cmfe-15.2015.22
ID  - Wang2015/07
ER  -