An Efficient Separation of Pyrroloquinoline Quinone Using Chemical Complexation Extraction

DOI

10.2991/cmfe-15.2015.20How to use a DOI?

Keywords

Nature Products; Pyrroloquinoline quinone; Separation; Chemical Complexation Extraction

Abstract

Pyrroloquinoline quinone (PQQ), an important natural product, is biosynthesized by Gluconobacter oxydans. In order to prepare the product in large scale, a rapid and selective chemical complexation extraction (CCE) is proposed to separate and purify PQQ from fermentation broth. In this work, the effect of extraction solvent system, stripping agent, and inorganic ion on the extraction were investigated, respectively. As a result, trioctylamine-octanol system showed the most extraction constants (log Kex = 5.07). Ammonia water was the most stripping agent (log Kex = 4.85). Several inorganic salts were determined to have negative effect on the extraction, which suggest that inorganic salts should be removed from broth before extraction. Finally, the optimal extraction condition is Trioctylamine-octanol system (1:1, v/v), 28 °C, and pH=6.5, which leads to more than 90% of extraction rate. In conclusion, trioctylamine-octanol system and ammonia water showed excellent extraction efficiency, which can be used to prepare PQQ.

Copyright

© 2015, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - XuePeng Yang
AU  - Panpan Li
AU  - Fangfang Wang
AU  - Jianbin Ye
AU  - Ke Ma
AU  - Duobin Mao
PY  - 2015/07
DA  - 2015/07
TI  - An Efficient Separation of Pyrroloquinoline Quinone Using Chemical Complexation Extraction
BT  - Proceedings of the International Conference on Chemical, Material and Food Engineering
PB  - Atlantis Press
SP  - 79
EP  - 82
SN  - 2352-5401
UR  - https://doi.org/10.2991/cmfe-15.2015.20
DO  - 10.2991/cmfe-15.2015.20
ID  - Yang2015/07
ER  -