Influence of Anthropogenic Chemical Factors on Immune Regulation in Petrochemical Production Workers with Cardiovascular System Pathology
- DOI
- 10.2991/ahsr.k.220103.040How to use a DOI?
- Keywords
- anthropogenic chemical factors; immune regulation; petrochemical production workers; cardiovascular system pathology
- Abstract
171 employees exposed to industrial anthropogenic chemical factors were examined. The sample was divided according to the principle of the presence of the cardiovascular diseases (CVD) (study group – 115 people) and their absence (comparison group – 56 people). For all employees, a chemical-analytical study of biological media for benzene content was carried out by gas chromatography. The study of cell phagocytosis was carried out using formalinized sheep erythrocytes. The content of IgG specific to benzene was determined by the allergosorbent method. Phenotyping of CD lymphocytes was performed on a flow cytometer by membrane immunofluorescence using a panel of labeled monoclonal antibodies (MoAbs) to membrane CD receptors. Determination of the TNFR, p53, Bcl-2, bax expression level was carried out using the corresponding MoAbs. Determination of total IgE, CA 19-9, PSA, interleukin-6, VEGF was performed by enzyme-linked immunosorbent assay (EIA method). The chemical and analytical studies identified an excess of the benzene content. Immunological studies revealed an increase in the index of phagocytic activity of cells. There were also significant changes in the CD indices of absolute and relative lymphocytes: the level of CD16+ cells of effectors increased, the level of CD19+, CD25+, CD95+ lymphocytes decreased, T-suppressor lymphocytes CD127- were activated, there was an increase in Annexin V-FITC + 7ADD-negative cells, TNFR, p53, bcl-2, bax. There was an increase in the general and specific sensitization of the body, as well as the level of tumor markers CA 19-9 and PSA, interleukin-6 and vascular endothelial growth factor VEGF. Significant changes in immunological parameters and the function of programmed cell death with simultaneous activation of sensitization processes reflected the peculiarities of the immune profile. These peculiarities formed disorders of the cardiovascular system functioning. Moreover, the increased level of specific sensitization to benzene emphasized the priority influence on the process of forming the workers’ labour experience along with the age factor.
- Copyright
- © 2022 The Authors. Published by Atlantis Press International B.V.
- Open Access
- This is an open access article under the CC BY-NC license.
Cite this article
TY - CONF AU - A.S. Shirinkina AU - I.N. Alikina AU - O.V. Dolgikh PY - 2022 DA - 2022/01/17 TI - Influence of Anthropogenic Chemical Factors on Immune Regulation in Petrochemical Production Workers with Cardiovascular System Pathology BT - Proceedings of the Conference on Health and Wellbeing in Modern Society (CHW 2021) PB - Atlantis Press SP - 199 EP - 203 SN - 2468-5739 UR - https://doi.org/10.2991/ahsr.k.220103.040 DO - 10.2991/ahsr.k.220103.040 ID - Shirinkina2022 ER -