Screen of Chryseobacterium CHANGBAI-2 strain and Identification of the Antifungal Antibiotic from Its Fermentation Broth
- DOI
- 10.2991/bst-17.2018.9How to use a DOI?
- Keywords
- Chryseobacterium, Screen, Antibiotic, identification, Fermentation broth
- Abstract
CHANGBAI-2 actinomycete strain was obtained by separating different kinds of soils from the nature. Moreover, the morphology, cultural characteristics, physiological-biochemical characteristics and 16S rDNA sequences of this strain were studied. A phylogenetic tree was constructed by comparing with the published 16S rDNA sequences of the related species and showed 99% identity of nucleotide sequence of 16S rDNA with Chryseobacterium. The antifungal substance produced by CHANGBAI-2 was purified by means of macro-porous adsorbent resin, and the crystal of the antibiotic with high purity was got. In this paper, the methods of purification by adsorbing of microporous adsorbent resin and detection by high performance liquid chromatography with mass spectrum (HPLC-MS) were established. The study would be laid a good foundation for its physical-chemical properties.
- Copyright
- © 2018, the Authors. Published by Atlantis Press.
- Open Access
- This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).
Cite this article
TY - CONF AU - Ning Yang AU - Yong Wang AU - Yu-Bo Wang AU - Li Zhang AU - Yong Lu AU - Quan-Jie Chen AU - Wen-Ge Zhang AU - Ming-Shan Ji PY - 2018/02 DA - 2018/02 TI - Screen of Chryseobacterium CHANGBAI-2 strain and Identification of the Antifungal Antibiotic from Its Fermentation Broth BT - Proceedings of the 2017 2nd International Conference on Biological Sciences and Technology (BST 2017) PB - Atlantis Press SP - 56 EP - 61 SN - 2468-5747 UR - https://doi.org/10.2991/bst-17.2018.9 DO - 10.2991/bst-17.2018.9 ID - Yang2018/02 ER -