Proceedings of the 2017 2nd International Conference on Biological Sciences and Technology (BST 2017)

Screen of Chryseobacterium CHANGBAI-2 strain and Identification of the Antifungal Antibiotic from Its Fermentation Broth

Authors
Ning Yang, Yong Wang, Yu-Bo Wang, Li Zhang, Yong Lu, Quan-Jie Chen, Wen-Ge Zhang, Ming-Shan Ji
Corresponding Author
Ning Yang
Available Online February 2018.
DOI
10.2991/bst-17.2018.9How to use a DOI?
Keywords
Chryseobacterium, Screen, Antibiotic, identification, Fermentation broth
Abstract

CHANGBAI-2 actinomycete strain was obtained by separating different kinds of soils from the nature. Moreover, the morphology, cultural characteristics, physiological-biochemical characteristics and 16S rDNA sequences of this strain were studied. A phylogenetic tree was constructed by comparing with the published 16S rDNA sequences of the related species and showed 99% identity of nucleotide sequence of 16S rDNA with Chryseobacterium. The antifungal substance produced by CHANGBAI-2 was purified by means of macro-porous adsorbent resin, and the crystal of the antibiotic with high purity was got. In this paper, the methods of purification by adsorbing of microporous adsorbent resin and detection by high performance liquid chromatography with mass spectrum (HPLC-MS) were established. The study would be laid a good foundation for its physical-chemical properties.

Copyright
© 2018, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

Download article (PDF)

Volume Title
Proceedings of the 2017 2nd International Conference on Biological Sciences and Technology (BST 2017)
Series
Advances in Biological Sciences Research
Publication Date
February 2018
ISBN
978-94-6252-472-9
ISSN
2468-5747
DOI
10.2991/bst-17.2018.9How to use a DOI?
Copyright
© 2018, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

Cite this article

TY  - CONF
AU  - Ning Yang
AU  - Yong Wang
AU  - Yu-Bo Wang
AU  - Li Zhang
AU  - Yong Lu
AU  - Quan-Jie Chen
AU  - Wen-Ge Zhang
AU  - Ming-Shan Ji
PY  - 2018/02
DA  - 2018/02
TI  - Screen of Chryseobacterium CHANGBAI-2 strain and Identification of the Antifungal Antibiotic from Its Fermentation Broth
BT  - Proceedings of the 2017 2nd International Conference on Biological Sciences and Technology (BST 2017)
PB  - Atlantis Press
SP  - 56
EP  - 61
SN  - 2468-5747
UR  - https://doi.org/10.2991/bst-17.2018.9
DO  - 10.2991/bst-17.2018.9
ID  - Yang2018/02
ER  -