Proceedings of the 2022 Brawijaya International Conference (BIC 2022)

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Combination Therapy of Green Tea and Green Coffee on Improving Cardiomyocyte Metabolism Through Increased Expression of AMPK and AKT Genes in Metabolic Syndrome Model Rats

Authors
Indah Nur Chomsy1, *, Mohammad Saifur Rohman2, Husnul Khotimah3, Nashi Widodo4, Nur Ida Panca Nugrahini5
1Doctoral Program of Medical Science, Faculty of Medicine, University of Brawijaya, Malang, 65145, Indonesia
2Department of Cardiology and Vascular Medicine, Saiful Anwar General Hospital, Faculty of Medicine, University of Brawijaya, Malang, 65145, Indonesia
3Laboratory of Pharmacology, Faculty of Medicine, University of Brawijaya, Malang, 65145, Indonesia
4Biology Department, Faculty of Mathematics and Natural Sciences, Brawijaya University, Malang, East Java, 65145, Indonesia
5Doctoral Program of Food Sciences, Department of Food Science and Biotechnology, Faculty of Agricultural Technology, University of Brawijaya, Malang, 65145, Indonesia
*Corresponding author. Email: indahncy@student.ub.ac.id
Corresponding Author
Indah Nur Chomsy
Available Online 30 April 2023.
DOI
10.2991/978-94-6463-140-1_48How to use a DOI?
Keywords
AMP-activated protein kinase; Protein Kinase B; Metabolic syndrome; metformin; green tea and coffee extract
Abstract

Metabolic syndrome (MetS) is a set of risk factors for metabolic abnormalities that can lead to dysfunction of organs, one of which is the heart. Each risk factor component of MetS represents a significant economic burden for patients, health plans, and society at large. One of the cardiovascular diseases (CVD) found is decrease in heart contractility. This cardiac dysfunction begins with abnormal expression of several proteins involved in lipid and glucose metabolism pathways, such as AMP-activated protein kinase (AMPK) and Protein Kinase B (Akt). One of the efforts to increase the use of environmentally friendly and sustainable resources, the use of herbs is the main focus in various treatments, one of which is MetS. This study aims to determine the effect of combination therapy of green tea and green coffee with metformin on cell metabolism pathways through the expression of AMPK and AKT genes in the heart of MetS model rats. Rats model were treated with metformin (MFN) 100 mg/kgbw, and/or green tea and green coffee extract (GTCE and/or COMB) 300 mg/kgbw and 200 mg/kgbw respectively. Rat hearts were isolated and analyzed by reverse-transcriptase PCR method using AMPK and AKT primers. The results showed that there was a significant difference in AMPK gene expression (p-value = 0.000) and AKT (p-value = 0.002) between all groups. The correlation between the two shows that they have a correlation with each other. This study showed that the combination therapy of green tea and green coffee with metformin was able to improve cell metabolic pathways by increasing the expression of AMPK and AKT genes in the heart of MetS model rats.

Copyright
© 2023 The Author(s)
Open Access
Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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Volume Title
Proceedings of the 2022 Brawijaya International Conference (BIC 2022)
Series
Advances in Economics, Business and Management Research
Publication Date
30 April 2023
ISBN
978-94-6463-140-1
ISSN
2352-5428
DOI
10.2991/978-94-6463-140-1_48How to use a DOI?
Copyright
© 2023 The Author(s)
Open Access
Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

Cite this article

risenwbib
TY  - CONF
AU  - Indah Nur Chomsy
AU  - Mohammad Saifur Rohman
AU  - Husnul Khotimah
AU  - Nashi Widodo
AU  - Nur Ida Panca Nugrahini
PY  - 2023
DA  - 2023/04/30
TI  - Combination Therapy of Green Tea and Green Coffee on Improving Cardiomyocyte Metabolism Through Increased Expression of AMPK and AKT Genes in Metabolic Syndrome Model Rats
BT  - Proceedings of the 2022 Brawijaya International Conference (BIC 2022)
PB  - Atlantis Press
SP  - 477
EP  - 487
SN  - 2352-5428
UR  - https://doi.org/10.2991/978-94-6463-140-1_48
DO  - 10.2991/978-94-6463-140-1_48
ID  - Chomsy2023
ER  -