Expression and Sequence Analysis of HTH in Blackberry (Rubus Spp.)

DOI

10.2991/bbbs-18.2018.1How to use a DOI?

Keywords

Rubus spp, HTH, Cloning and expression, Light irradiation.

Abstract

To better understand the molecular mechanisms of cuticle wax formation in blackberry, the waxy synthetic related gene HTH was cloned from blackberry. The open reading frame of this gene was 1785 bp, encoding 594 amino acid residues with a molecular weight of 64.6 kD and a theoretical isoelectric point of 9.39. Its amino acid sequence has the highest identity with strawberry. The results of real-time quantitative PCR showed that the expression level of HTH gene decrease rapidly with the maturity of the fruit. The expression level of HTH decreased with the increase of red or blue light intensity. The lowest expression of HTH gene was under pure blue light treatment.

Copyright

© 2018, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Xunju Liu
AU  - Min Zhou
AU  - Yan Wang
AU  - Haoru Tang
AU  - Qing Chen
PY  - 2018/04
DA  - 2018/04
TI  - Expression and Sequence Analysis of HTH in Blackberry (Rubus Spp.)
BT  - Proceedings of the 2018 International Workshop on Bioinformatics, Biochemistry, Biomedical Sciences (BBBS 2018)
PB  - Atlantis Press
SP  - 1
EP  - 5
SN  - 2468-5747
UR  - https://doi.org/10.2991/bbbs-18.2018.1
DO  - 10.2991/bbbs-18.2018.1
ID  - Liu2018/04
ER  -