Antibacterial Activity of Single Garlic Against Salmonella typhi Lipopolysacaride in Vivo in Mus musculus

DOI

10.2991/978-94-6463-060-2_15How to use a DOI?

Keywords

Antibacterial; Black garlic; Lipopolysaccharide; TNF-α

Abstract

Black garlic is a product of fermented garlic. According to previous research, black garlic has anti-inflammatory properties such as against Salmonella typhi. S. typhi has lipopolysaccharide (LPS) which will activate inflammatory cytokine mediators such as Tumor necrosis factor-alpha (TNF-α). TNF- is one of the major cytokines in the acute inflammatory response. This study aims to determine the anti-inflammatory properties and concentration of single black garlic extract against S. typhi lipopolysaccharide in vivo in mice. This study used an experimental method with a posttest only research design with control group¸ using BALB/C white male mice which were divided into 5 groups, namely negative control (NC), positive control (PC), treatment 1 (T1) was given 25% extract. Treatment 2 (T2) was given 50% extract, and treatment 3 (T3) was given 75% extract. Lipopolysaccharide induction of 0.5 mg/BW was carried out for 3 days after 14 days of extract administration. Blood samples were taken 24 h after LPS induction. The average result of TNF- levels was obtained in the PC group with the largest 8.43 pg/ml, the lowest NC group 4.69 pg/ml, of all treatment groups, the lowest T3 group 6.08 pg/ml. The results of data analysis showed p = 0.000 (p < 0.05). This indicates that there are significant differences in TNF-α levels in all study groups. It was concluded that a single black garlic extract has anti-bacterial properties because it can reduce TNF- levels in LPS-induced mice with the most effective concentration of 75%.

Copyright

© 2023 The Author(s)

Open Access

Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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TY  - CONF
AU  - Lia Siti Halimah
AU  - Luthfi Nurlela
AU  - Eka Noneng Nawangsih
AU  - Reni Farenia
AU  - Dhisa Ridzkianursepta
PY  - 2022
DA  - 2022/12/16
TI  - Antibacterial Activity of Single Garlic Against Salmonella typhi Lipopolysacaride in Vivo in Mus musculus
BT  - Proceedings of The 13th Annual Scientific Conference of Medical Faculty, Universitas Jenderal Achmad Yani (ASCMF 2022)
PB  - Atlantis Press
SP  - 105
EP  - 110
SN  - 2468-5739
UR  - https://doi.org/10.2991/978-94-6463-060-2_15
DO  - 10.2991/978-94-6463-060-2_15
ID  - Halimah2022
ER  -