Molecular Cloning and Structure Analysis Of MpYop1, a Membrane Protein in Monascus purpureus

DOI

10.2991/aeece-15.2015.148How to use a DOI?

Keywords

Membrane; Transmembrane helix; cloning; Monascus purpureus; endoplasmic reticulum.

Abstract

In all eukaryotes, the peripheral endoplasmic reticulum (ER) branches out of the nuclear envelope as a membrane network of interconnected tubules and membrane sheets with a single lumen. In yeast, Rtn1p and Yop1p are major, redundant components required to maintain the tubular ER under normal circumstances. In this work, we have investigated the homolog of the yeast Yop1 protein in Monascus purpureus. An M. purpureus cDNA library was constructed, and the MpYop1 cDNA was isolated from the cDNA library by random sequencing method. The cDNA was 1008 bp in length, contains a predicted 516 bp ORF that encodes 171 amino acids, the gene was designated MpYop1. The deduced amino acid sequence showed homology to Yop1 from fungi. The TM topology model of MpYop1 was built, the secondary structure is similar to yeast Yop1p. MpYop1 contains four transmembrane -helices connected by a cytoplasmic loop and two extracellar loop, both the N and C termini are located in the cytoplasm side of ER membrane. According to functions of Yop1 in Yeast, we implicated that the MpYop1 would involved in stabilization of tubule ER.

Copyright

© 2015, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Haoming Li
AU  - Lan Gao
PY  - 2015/09
DA  - 2015/09
TI  - Molecular Cloning and Structure Analysis Of MpYop1, a Membrane Protein in Monascus purpureus
BT  - Proceedings of the International Conference on Advances in Energy, Environment and Chemical Engineering
PB  - Atlantis Press
SP  - 731
EP  - 734
SN  - 2352-5401
UR  - https://doi.org/10.2991/aeece-15.2015.148
DO  - 10.2991/aeece-15.2015.148
ID  - Li2015/09
ER  -