Journal of Epidemiology and Global Health

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Volume 7, Issue 3, September 2017, Pages 175 - 180

Frequency of multi-drug resistance and mutations in Mycobacterium tuberculosis isolates from Punjab state of India

Authors
Ritu Singhala, Jyoti Aroraa, Grish C. Saha, Manpreet Bhallaa, Rohit Sarinb, Vithal Prasad Myneedua, *, tbmicro@gmail.com
aDepartment of Microbiology, National Institute of Tuberculosis and Respiratory Diseases, New Delhi 110030, India
bDepartment of Tuberculosis and Respiratory Diseases, National Institute of Tuberculosis and Respiratory Diseases, New Delhi 110030, India
*Corresponding author. at Department of Microbiology, National Institute of Tuberculosis and Respiratory Diseases, AurobindoMarg, New Delhi 110030, India
Corresponding Author
Vithal Prasad Myneedutbmicro@gmail.com
Received 18 February 2017, Revised 14 April 2017, Accepted 12 May 2017, Available Online 24 May 2017.
DOI
10.1016/j.jegh.2017.05.002How to use a DOI?
Keywords
MDR suspect; Genotype MTBDRplus; Mutation
Abstract

Data regarding prevalence of multi-drug resistant tuberculosis (MDR-TB) and associated common mutations is scarce from Punjab region. The study was designed to determine rate of MDR-TB among presumptive MDR-TB from Punjab and mutation patterns using GenoType MTBDRplus assay. Total of 812 consecutive sputum samples were received from January 2012 to July 2013, from 14 districts of Punjab at the National Reference Laboratory at New Delhi for diagnosis of MDR-TB as hand holding activity. Presumptive MDR-TB patients were identified on basis of criterion B defined by the programme. Smear positive and negatives patients were found to be 636/798 (79.7%) and 162/ 798 (20.3%) respectively. Total of 606 GenoType MTBDRplus tests were conducted and mutations in rpoB, kat G and inhA genes analyzed. Total of 94/606 (15.5%), 43/606 (7.1%) and 40/606 (6.6%) were found to be RIF and INH resistant, mono-RIF resistant and 40/606 (6.6%) mono-INH resistant respectively. Commonest known mutation for RIF in rpoB gene and INH in kat G gene was S531L (80/ 137; 58.4%) and S315T1 (119/134; 88.8%) respectively. Mutations in inhA were found in 21/134 (15.7%) strains. Average turn-around time (TAT) for dispatch of result toPunjab was 4.6 days. Prevalence of RIF resistance in Punjab was found to be 22.6%. Common mutations for RIF and INH were similar to that in other regions of country. GenoType MTBDRplus was found to be useful assay for rapid detection of MDR-TB, responsible for determining better management of MDR-TB patients under the programme.

Copyright
© 2017 Ministry of Health, Saudi Arabia. Published by Elsevier Ltd.
Open Access
This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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<Previous Article In Issue
Next Article In Issue>
Journal
Journal of Epidemiology and Global Health
Volume-Issue
7 - 3
Pages
175 - 180
Publication Date
2017/05/24
ISSN (Online)
2210-6014
ISSN (Print)
2210-6006
DOI
10.1016/j.jegh.2017.05.002How to use a DOI?
Copyright
© 2017 Ministry of Health, Saudi Arabia. Published by Elsevier Ltd.
Open Access
This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

Cite this article

risenwbib
TY  - JOUR
AU  - Ritu Singhal
AU  - Jyoti Arora
AU  - Grish C. Sah
AU  - Manpreet Bhalla
AU  - Rohit Sarin
AU  - Vithal Prasad Myneedu
PY  - 2017
DA  - 2017/05/24
TI  - Frequency of multi-drug resistance and mutations in Mycobacterium tuberculosis isolates from Punjab state of India
JO  - Journal of Epidemiology and Global Health
SP  - 175
EP  - 180
VL  - 7
IS  - 3
SN  - 2210-6014
UR  - https://doi.org/10.1016/j.jegh.2017.05.002
DO  - 10.1016/j.jegh.2017.05.002
ID  - Singhal2017
ER  -