P70 Effects of Different Vitamin D Status on Mechanical, Pharmacological and Histological Characteristics of Coronary Arterioles in Male Rat Model

DOI

10.2991/artres.k.191224.101How to use a DOI?

Abstract

Objectives: The cardiovascular/CV risk is higher in males. To know better these increased risks, our aim was to investigate the effects of different vitamin D/VD status on mechanical, pharmacological and histological characteristics of coronary arterioles in male rat model.

Methods: In male Wistar-rats we induced different VD status: “Group C” got 1000 IU VD containing rat chow and VD-supplementation, “Group D-” got less, than 5 IU VD containing rat chow to model the VD deficient/VDD status. At the end of the experiment we prepared intramural, small coronary arteriole segments from the LAD. With microangiometer, we measured the inner and outer diameters, TXA2-vasoconstriction, adenosine-vasorelaxation, and the passive diameter. Histopathological examinations were taken.

Results: Inner and outer radius were significantly decreased, wall thickness and wall-thickness/lumen diameter ratio were significantly increased in group D-, while the wall-cross-sectional area and the intima-media area% were not different. Tangential wall stress and incremental elastic modulus were significantly reduced in group D-. TXA2-vasoconstriction significantly lower in group D-. In the point of adenosine-induced vasorelaxation, in group D-only the highest dose of adenosine could relax the vessels. eNOS-expression was not differ, SMA-expression was significantly decreased in group D-.

Conclusion: The characteristics of the VDD group’s coronary arterioles had changed. The results refer to development of inner eutrophic remodelling and changed wall structure. These changes can cause increased vascular resistance and diminished coronary perfusion which as a result lead to the deterioration of the heart’s blood supply. These mechanisms can contribute to the increased incidence of the CV diseases in VDD.

Copyright

© 2019 Association for Research into Arterial Structure and Physiology. Publishing services by Atlantis Press International B.V.

Open Access

This is an open access article distributed under the CC BY-NC 4.0 license (http://creativecommons.org/licenses/by-nc/4.0/).

TY  - JOUR
AU  - Reka Eszter Sziva
AU  - Zoltan Fontanyi
AU  - Eva Pal
AU  - Leila Hadjadj
AU  - Anna Monori-Kiss
AU  - Eszter Maria Horvath
AU  - Rita Benko
AU  - Attila Magyar
AU  - Andrea Heinzlmann
AU  - Zoltan Benyo
AU  - Gyorgy L. Nadasy
AU  - Szabolcs Varbiro
AU  - Bálint Bányai
PY  - 2020
DA  - 2020/02/17
TI  - P70 Effects of Different Vitamin D Status on Mechanical, Pharmacological and Histological Characteristics of Coronary Arterioles in Male Rat Model
JO  - Artery Research
SP  - S114
EP  - S114
VL  - 25
IS  - Supplement 1
SN  - 1876-4401
UR  - https://doi.org/10.2991/artres.k.191224.101
DO  - 10.2991/artres.k.191224.101
ID  - Sziva2020
ER  -