9.1 ULTRASOUND CHARACTERIZATION OF CARDIOVASCULAR ALTERATIONS IN YOUNG OB/OB MICE

DOI

10.1016/j.artres.2016.10.068How to use a DOI?

Abstract

Obesity is associated with diabetes and an increased cardiovascular risk. Leptin-deficient mice (ob/ob) are used as model of metabolic disease: they are characterized by obesity and insulin-resistance. Aim of this work is to identify early cardiovascular alterations in young ob/ob mice using micro-ultrasound imaging.

Sixteen wild-type (wt) and eleven ob/ob male mice (8 wo,C57BL6) were studied. B-mode and PW-Doppler images were acquired with a micro-ultrasonographic system (Vevo2100) for assessing cardiovascular biomarkers. Left ventricular mass (LVmass), cardiac output (CO), ejection fraction (EF), stroke volume (SV), fractional shortening (FS) and E/A ratio were measured. Mean diameter (Dm_abd and Dm_car), relative distension (relD_abd and relD_car) and pulse wave velocity (PWVabd and PWVcar) were obtained for both abdominal aorta and common carotid. As regards renal microcirculation, renal resistivity and pulsatility index (RI and PI) were assessed. The ratio between grey-levels related to liver and kidney (HR_ratio) was used as index of hepatic steatosis grade.

ob/ob mice had higher glycemia levels (ob/ob:296±42mg/dl, wt:149±23mg/dl, p<0.01) and higher weight (ob/ob: 44.2g, wt: 31.1g, p<0.01). relD abd values were lower for ob/ob mice than for wt ones (ob/ob:18.6±4.1%, wt:23.8±3.3%, p<0.01) the ob/ob group presented also higher PWV (ob/ob:2.11±0.69m/s, wt:1.73±0.43m/s, p<0.05), RI (ob/ob:0.71±0.05, wt:0.64±0.06m/s, p<0.01) and PI (ob/ob:1.15±0.17, wt:0.97±0.12m/s, p<0.01) values. As concerns hepatic steatosis, there was a difference in HR ratio evaluations (ob/ob:1.27±0.26, wt:0.79±0.17, p<0.01).!

Young ob/ob mice have a reduced abdominal aorta distension capability and a higher value of stiffness for this vessel. Moreover, starting from young age, parameters related to renal microcirculation and hepatic fat accumulation are altered.

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TY  - JOUR
AU  - Nicole Di Lascio
AU  - Claudia Kusmic
AU  - Francesco Stea
AU  - Francesco Faita
PY  - 2016
DA  - 2016/11/24
TI  - 9.1 ULTRASOUND CHARACTERIZATION OF CARDIOVASCULAR ALTERATIONS IN YOUNG OB/OB MICE
JO  - Artery Research
SP  - 68
EP  - 68
VL  - 16
IS  - C
SN  - 1876-4401
UR  - https://doi.org/10.1016/j.artres.2016.10.068
DO  - 10.1016/j.artres.2016.10.068
ID  - DiLascio2016
ER  -