P173 COUPLED NITROSO-SULFIDE SIGNALIZATION TRIGGERS SPECIFIC VASOACTIVE EFFECTS IN INTRARENAL ARTERIES OF PATIENTS WITH ARTERIAL HYPERTENSION

DOI

10.1016/j.artres.2017.10.121How to use a DOI?

Abstract

In normotensive conditions, it has been confirmed that S-nitrosothiols, as a source of NO, interact with hydrogen sulfide (H₂S) and create new substance/s with specific vasoactive effects. This interaction could represent new regulator pathway also in hypertension. The aim of the study was to investigate the vasoactive effects of H₂S, GSNO, and products of H₂S/GSNO interaction in lobar arteries isolated from kidney after nephrectomy of patients suffering from arterial hypertension.

Changes in isometric tension after pre-contraction were evaluated. Acetylcholine- induced vasorelaxation was significantly reduced compared to the effect induced by exogenous NO donor, sodium nitroprusside, probably suggesting an endothelium dysfunction. While 1 μmol/l Na₂S had a minimal effect on the vascular tone, 20 μmol/l evoked a slight vasorelaxation. GSNO at 0.1 μmol/l induced vasorelaxation which was significantly smaller compared to the effect induced by 1μmol/l. The mixture of GSNO (0.1 μmol/l) and Na₂S (1 μmol/l) induced significantly higher vasorelaxation compared to GSNO (0.1 μmol/l) alone only in 5^th minute without the differences in the speed.

On the other hand, the mixture prepared from higher concentrations of GSNO (1 μmol/l) and Na₂S (10 μmol/l) induced a significantly higher (in 1st, 2nd, 5th, 10th minute) and faster vasorelaxation compared to the effect induced by GSNO (1 μmol/l) alone.

In conditions of arterial hypertension H₂S in interaction with GSNO regulated a vasoconstrictor-increased arterial tone towards of more pronounced vasorelaxation compared to GSNO alone. We confirmed for the first time that specific vasoactive effects of coupled nitroso-sulfide signalization were triggered also in human arterial tissue.

Open Access

This is an open access article distributed under the CC BY-NC license.

TY  - JOUR
AU  - Sona Cacanyiova
AU  - Andrea Berenyiova
AU  - Frantisek Kristek
AU  - Marian Grman
AU  - Karol Ondrias
AU  - Jan Breza
AU  - Jan Breza
PY  - 2017
DA  - 2017/12/06
TI  - P173 COUPLED NITROSO-SULFIDE SIGNALIZATION TRIGGERS SPECIFIC VASOACTIVE EFFECTS IN INTRARENAL ARTERIES OF PATIENTS WITH ARTERIAL HYPERTENSION
JO  - Artery Research
SP  - 85
EP  - 85
VL  - 20
IS  - C
SN  - 1876-4401
UR  - https://doi.org/10.1016/j.artres.2017.10.121
DO  - 10.1016/j.artres.2017.10.121
ID  - Cacanyiova2017
ER  -